Detecting Cellulase Penetration Into Corn Stover Cell Walls by Immuno-Electron Microscopy
Detecting Cellulase Penetration Into Corn Stover Cell Walls by Immuno-Electron Microscopy
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DOI：
10.1002/bit.22281
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发表时间：
2009-06-15
影响因子：
3.8
通讯作者：
Himmel, Michael E.
中科院分区：
文献类型：
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作者：
Donohoe, Bryon S.;Selig, Michael J.;Himmel, Michael E.
通常，预处理被设计为增强纤维素对酶的可及性，从而允许更有效的转化。在这项研究中，我们已经检测了在轻度、中度和高度苛刻的稀硫酸预处理后，商业酶制剂（Spezyme CP）中存在的主要纤维素酶渗透到玉米茎细胞壁中。里氏木霉酶Cel7A（CBH I）和Cel7B（EG I）以及细胞壁基质组分木聚糖和木质素通过免疫透射电子显微镜（TEM）使用酶和聚合物特异性抗体在消化的玉米秸秆细胞壁内可视化。启用低强度稀酸预处理（100 ℃下20分钟）
In general, pretreatments are designed to enhance the accessibility of cellulose to enzymes, allowing for more efficient conversion. in this study, we have detected the penetration of major cellulases present in a commercial enzyme preparation (Spezyme CP) into corn stern cell walls following mild-, moderate- and high-severity dilute sulfuric acid pretreatments. The Trichoderma reesei enzymes, Cel7A (CBH I) and Cel7B (EG I), as well as the cell wall matrix components xylan and lignin were visualized within digested corn stover cell walls by immuno transmission electron microscopy (TEM) using enzyme- and polymer-specific antibodies. Low severity dilute-acid pretreatment (20 min at 100 degrees C) enabled