Electrochemiluminescent (ECL) biosensor for Burkholderia pseudomallei based on cobalt-doped MOF decorated with gold nanoparticles and N-(4-aminobutyl)-N-(ethylisoluminol)
Electrochemiluminescent (ECL) biosensor for Burkholderia pseudomallei based on cobalt-doped MOF decorated with gold nanoparticles and N-(4-aminobutyl)-N-(ethylisoluminol)
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DOI：
10.1007/s00604-022-05402-6
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发表时间：
2022-08
影响因子：
5.7
通讯作者：
Yuexin Wang;Rui Chen;Bo Shen;Cai-xia Li;Junman Chen;Yanshuang Wang;Shen Tian;Xuemiao Li
中科院分区：
文献类型：
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作者：
Yuexin Wang;Rui Chen;Bo Shen;Cai-xia Li;Junman Chen;Yanshuang Wang;Shen Tian;Xuemiao Li
报道了一种由钴掺杂金属有机骨架（Co-MOF）、金纳米粒子（AuNP）和N-（4-氨基丁基）-N-（乙基异鲁米诺）（ABEI）组成的多功能催化纳米材料（Co-MOF@AuNP@ABEI）。Co-MOF@AuNP@ABEI具有良好的协同效应和零距离催化特性，有利于提高电致发光（ECL）生物传感器的检测灵敏度。与ECL体系和三维磁行走纳米机器扩增策略耦合后，Co-MOF@AuNP@ABEI可实现对类鼻疽伯克霍尔德菌的超灵敏ECL检测，检测限（LOD）为60.3 aM，比单独的ECL体系低2和4个数量级（4.97 fM）和单个步行纳米机器（340 fM），并且上级先前报告中的病原菌分析。此外，拟定的ECL检测系统用于测定B.血清样本中的假鼻疽毒素低至9.0 CFU mL−1。测定了5种B.假鼻疽加标血清样品的检出率分别为4.02%、0.84%、0.84%、1.55%和0.21%。电化学发光生物传感器检测B.加标血清标本的检出率为93.63 ~ 107.83%。因此，本工作证明了所开发的具有协同和零距离催化特性的多功能催化纳米材料可以作为优异的ECL信号报告子，提高ECL生物传感器的检测灵敏度。
A multifunctional catalytic nanomaterial (Co-MOF@AuNP@ABEI) composed of cobalt-doped metal–organic frameworks (Co-MOF), gold nanoparticles (AuNP), andN-(4-aminobutyl)-N-(ethylisoluminol) (ABEI) is reported. Co-MOF@AuNP@ABEI exhibits high synergistic and zero-distance catalytic properties, which are beneficial to the improvement of the detection sensitivity of an electrochemiluminescent (ECL) biosensor. After coupling with the ECL system and 3D magnetic walking nanomachine amplification strategy, the Co-MOF@AuNP@ABEI can achieve an ultrasensitive ECL assay ofBurkholderia pseudomalleiwith the limit of detection (LOD) of 60.3 aM, which is 2 and 4 orders of magnitude lower than individual ECL system without the nanomachine (4.97 fM) and individual walking nanomachine (340 fM), and superior to the pathogenic bacteria analyses in the previous report. Moreover, the LOD of the proposed ECL detection system for the determination ofB. pseudomalleiin serum sample was as low as 9.0 CFU mL−1. The relative standard deviations (RSD) of ECL intensity for the detection of fiveB. pseudomallei-spiked serum samples were 4.02%, 0.84%, 0.84%, 1.55%, and 0.21%, respectively. The recoveries of the ECL biosensor for the detection ofB. pseudomalleiDNA-spiked serum samples were 93.63 ~ 107.83%. Therefore, this work demonstrated that the developed multifunctional catalytic nanomaterial with synergistic and zero-distance catalytic properties can be used as excellent ECL signal reporter to improve the detection sensitivity of ECL biosensor.Graphical abstract