Genetic Diversity of Salt Tolerance in Miscanthus.
Genetic Diversity of Salt Tolerance in Miscanthus.
复制标题
DOI：
10.3389/fpls.2017.00187
复制
发表时间：
2017
影响因子：
5.6
通讯作者：
van der Linden CG
中科院分区：
文献类型：
--
作者：
Chen CL;van der Schoot H;Dehghan S;Alvim Kamei CL;Schwarz KU;Meyer H;Visser RG;van der Linden CG
芒草是一种木质根茎C4草，可用作CO2中性生物燃料资源。它有潜力在盐碱地等边缘地区生长，避免与粮食作物争夺耕地。本研究旨在探索芒属植物耐盐性的遗传多样性，并揭示盐胁迫下提高产量的机制和性状。芒属植物70个基因型（包括芒属57个M. sinensis，5 M. sacchariflorus和8个杂交种）在盐水（150 mM NaCl）和正常生长条件下使用溶液培养系统评价耐盐性。地上部生长性状和离子浓度的分析表明，耐盐性在基因型间存在较大的变异。我们确定了在控制和盐条件下具有高生物量生产潜力的基因型，这些基因型可用于在边际盐条件下生长。几个相对耐盐的基因型有明显较低的Na+浓度和盐胁迫下表现出相对较高的K+/Na+比值，表明Na+排斥机制，以防止Na+积累在叶片中。其他基因型在盐条件下表现出有限的叶片扩展和生长速率的降低，这可能是渗透胁迫耐受性的指示。表现出潜在的不同耐盐机制的基因型可以作为旨在提高芒属植物耐盐性的育种计划的起始材料。
Miscanthus is a woody rhizomatous C4 grass that can be used as a CO2 neutral biofuel resource. It has potential to grow in marginal areas such as saline soils, avoiding competition for arable lands with food crops. This study explored genetic diversity for salt tolerance in Miscanthus and discovered mechanisms and traits that can be used to improve the yield under salt stress. Seventy genotypes of Miscanthus (including 57 M. sinensis, 5 M. sacchariflorus, and 8 hybrids) were evaluated for salt tolerance under saline (150 mM NaCl) and normal growing conditions using a hydroponic system. Analyses of shoot growth traits and ion concentrations revealed the existence of large variation for salt tolerance in the genotypes. We identified genotypes with potential for high biomass production both under control and saline conditions that may be utilized for growth under marginal, saline conditions. Several relatively salt tolerant genotypes had clearly lower Na+ concentrations and showed relatively high K+/Na+ ratios in the shoots under salt stress, indicating that a Na+ exclusion mechanism was utilized to prevent Na+ accumulation in the leaves. Other genotypes showed limited reduction in leaf expansion and growth rate under saline conditions, which may be indicative of osmotic stress tolerance. The genotypes demonstrating potentially different salt tolerance mechanisms can serve as starting material for breeding programs aimed at improving salinity tolerance of Miscanthus.