VOLUME-DEPENDENT AND NEM-STIMULATED K+, CL- TRANSPORT IS ELEVATED IN OXYGENATED SS, SC AND CC HUMAN RED-CELLS
VOLUME-DEPENDENT AND NEM-STIMULATED K+, CL- TRANSPORT IS ELEVATED IN OXYGENATED SS, SC AND CC HUMAN RED-CELLS
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DOI：
10.1016/0014-5793(86)80538-5
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发表时间：
1986-05-05
期刊：
影响因子：
3.5
通讯作者：
NAGEL, RL
中科院分区：
文献类型：
--
作者：
CANESSA, M;SPALVINS, A;NAGEL, RL
参与细胞体积调节的机制在SS、SC细胞中很重要，因为它们可能参与决定镰状细胞的程度以及致密细胞和不可逆转镰状细胞的产生。我们在这些细胞中研究了K+Cl−转运体对细胞肿胀的反应。我们发现Hb-SS、SC和CC红细胞具有较高的哇巴因抗性、氯离子依赖和NEM刺激的K+外流。相反，从布美他尼敏感的K+外流成分估算的Na+K+Cl−共转运在SS、SC和CC红细胞中没有显著差异。降低渗透压(300~220)和pH(8~7)可刺激SS、SC和CC红细胞耐(哇巴因+布美他尼)K+外流。渗透肿胀刺激的依赖氯−的K+外流与N EM刺激组分(r=0.8p<；0.001，n=22)和酸诱导的溶胀(r=0.969，p<；0.001，n=22)高度相关，表明它是由K+Cl−转运体驱动的。
Mechanisms involved in cell volume regulation are important in SS, SC cells as they might be involved in determining the extent of sickling and the generation of dense cells and irreversibly sickled cells. We have studied in these cells the response to cell swelling of the K+Cl−transporter. We found that Hb SS, SC and CC red cells have higher values of a ouabain‐resistant, chloride‐dependent and NEM‐stimulated K+efflux than AA red cells. In contrast, the Na+K+Cl−cotransport estimated from the bumetanide‐sensitive component of K+efflux was not significantly different in SS, SC and CC red cells. The (ouabain + bumetanide)‐resistantK+efflux from SS, SC and CC red cells was stimulated by cell swelling induced by reduction of the osmotic pressure (300 to 220 ) and pH (8 to 7) of the flux media (140 mM NaCl). The Cl−‐dependent K+efflux stimulated by osmotic swelling highly correlated with the NEM‐stimulated component (r= 0.8,p< 0.001,n= 22) and the acid‐pH‐induced swelling (r= 0.969,p< 0.001,n= 22), indicating that it is driven by the K+Cl−transporter.