Aromatic metabolism in plants. II. Enzymes of the shikimate pathway in suspension cultures of plant cells.
Aromatic metabolism in plants. II. Enzymes of the shikimate pathway in suspension cultures of plant cells.
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植物中的芳香代谢。
DOI：
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发表时间：
1966
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影响因子：
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通讯作者：
O. Gamborg
中科院分区：
文献类型：
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作者：
O. Gamborg
以大豆根、蜡豆根、玫瑰茎、芦笋茎、辣根叶柄、马铃薯块茎、荞麦下胚轴组织和绿豆子叶、根和下胚轴组织为材料，建立了液体悬浮培养。玫瑰和胭脂树培养不需要外部供应的生长调节剂。描述了无细胞提取物的培养和制备方法。检测细胞提取物的异柠檬酸脱氢酶（EC 1.1.1.42）、苹果酸脱氢酶（EC 1.1.1.37）、醌酸脱氢酶（EC 1.1.1.24）、脱氢醌酸脱氢酶（EC 4.2.1.10）、莽草酸脱氢酶（EC 1.1.1.25）、预苯酸脱氢酶（EC 1.1.1）、苯丙氨酸转氨酶（EC 2.6.1）和苯丙氨酸解氨酶（EC 4.3.1.5）。在绿豆培养物中检测到所有的酶。在所有培养物中均存在异柠檬酸脱氢酶和草酸脱氢酶、脱氢奎酸脱氢酶和苯丙氨酸解氨酶。在绿豆培养物和室内培养基中检测到预苯酸脱氢酶。
Liquid suspension cultures were established from tissues of soybean root, wax-bean root, rose stem, reseda stem, horseradish petiole, potato tuber, buckwheat hypocotyl, and of cotyledon, root, and hypocotyl from mung bean. The rose and the reseda cultures required no external supply of growth regulators. Methods of culture and preparation of cell-free extracts are described. Extracts from the cells were assayed for isocitrate dehydrogenase (EC 1.1.1.42), malate dehydrogenase (EC 1.1.1.37), quinate dehydrogenase (EC 1.1.1.24), dehydroquinate dehydratase (EC 4.2.1.10), shikimate dehydrogenase (EC 1.1.1.25), prephenate dehydrogenase (EC 1.1.1.), phenylalanine transaminase (EC 2.6.1.), and phenylalanine ammonia-lyase (EC 4.3.1.5). All of the enzymes were detected in the cultures from mung bean. The isocitrate and shikimate dehydrogenases, dehydroquinate dehydratase, and phenylalanine ammonia-lyase were present in all the cultures. The prephenate dehydrogenase was detected in the mung-bean cultures and the roo...