Influence of host cell infiltration on the glycolipid content of mouse brain tumors.
Influence of host cell infiltration on the glycolipid content of mouse brain tumors.
复制标题
宿主细胞浸润对小鼠脑肿瘤糖脂含量的影响。
DOI：
10.1046/j.1471-4159.1996.66052026.x
复制
发表时间：
1996
影响因子：
4.7
通讯作者：
Yohe,HC
中科院分区：
文献类型：
--
作者：
Seyfried,TN;el-Abbadi,M;Ecsedy,JA;Bai,HW;Yohe,HC
先前的研究表明，在体内生长的实体脑肿瘤中表达的一些鞘糖脂（GSL）的水平在从肿瘤制备的培养细胞中降低或检测不到。这种现象归因于组织培养环境的未知力量抑制糖脂合成或缺乏通常渗入体内生长的实体瘤的宿主细胞。为了进一步验证宿主细胞假说，我们检查了两种实验小鼠脑肿瘤（室管膜母细胞瘤和CT-2A）的宿主细胞标志物，这些肿瘤在C57 BL/6 J（B6）小鼠的侧腹中作为皮下实体瘤生长或作为体外培养细胞生长。标记物包括神经节苷脂N-羟乙酰神经氨酸（NeuGc）、GA 1（去唾液酸-GM 1）和Fc受体携带细胞。含NeuGc的神经节苷脂、GA 1和Fc受体由小鼠宿主免疫系统的巨噬细胞和淋巴样细胞表达，但通常不由小鼠神经细胞表达。体内生长的室管膜母细胞瘤和CT-2A肿瘤中Fc受体携带细胞的相对含量差异（分别为8.3%和16.8%）分别与含NeuGc神经节苷脂（25.5%和45.1%）和GA 1（8.5%和13.8%）的相对含量差异成比例。培养的肿瘤细胞系均不表达Fc受体、GA 1或含NeuGc的神经节苷脂。这些发现表明，非肿瘤性宿主浸润细胞（巨噬细胞）对体内生长的实体瘤的GSL组成有显著贡献。
Previous studies showed that levels of some glycosphingolipids (GSLs) expressed in solid brain tumors grown in vivo were reduced or undetectable in cultured cells prepared from the tumors. This phenomenon has been attributed either to suppressed glycolipid synthesis from unknown forces of the tissue culture environment or to the absence of host cells that normally infiltrate the solid tumors growing in vivo. To test further the host cell hypothesis, we examined host cell markers in two experimental mouse brain tumors, the ependymoblastoma and the CT‐2A, that were grown as subcutaneous solid tumors in the flank of C57BL/6J (B6) mice or as cultured cells in vitro. The markers included gangliosideN‐glycolylneuraminic acid (NeuGc), GA1 (asialo‐GM1), and Fc receptor‐bearing cells. NeuGc‐containing gangliosides, GA1, and Fc receptors are expressed by macrophages and lymphoid‐type cells of the mouse host immune system but are not normally expressed by mouse neural cells. Differences in the relative content of Fc receptor‐bearing cells in ependymoblastoma and CT‐2A tumors grown in vivo (8.3 and 16.8%, respectively) were proportional to differences in the relative content of NeuGc‐containing gangliosides (25.5 and 45.1%) and GA1 (8.5 and 13.8%), respectively. Neither cultured tumor cell line expressed Fc receptors, GA1, or NeuGc‐containing gangliosides. These findings suggest that non‐neoplastic host infiltrating cells (macrophages) contribute significantly to the GSL composition of solid tumors growing in vivo.