Dynamics of spontaneous activity in neocortical slices
Dynamics of spontaneous activity in neocortical slices
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DOI：
10.1016/s0896-6273(01)00518-9
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发表时间：
2001-12-06
期刊：
影响因子：
16.2
通讯作者：
Yuste, R
中科院分区：
文献类型：
--
作者：
Mao, BQ;Hamzei-Sichani, F;Yuste, R
人们对皮质微电路的活动流程知之甚少。我们使用钙成像以毫秒和单细胞分辨率重建小鼠视觉皮层未刺激切片中神经元群体的自发活动。我们发现第 5 层锥体细胞网络之间的自发活动相关。同步系综占据重叠的区域，通常共享神经元，并且被反复激活。神经元组也被连续激活多次。尽管自发放电在许多“自主活动”神经元中持续存在，但网络同步和顺序相关性被谷氨酸拮抗剂阻断。这种自主活动是周期性的，取决于超极化激活的阳离子 (H) 和持续的钠 (Na-p) 电流。我们得出的结论是，孤立的新皮质微电路产生自发活动，由内在机制和电路机制的组合介导，并且这种活动可以在时间上精确。
The flow of activity in the cortical microcircuitry is poorly understood. We use calcium imaging to reconstruct, with millisecond and single-cell resolution, the spontaneous activity of populations of neurons in unstimulated slices from mouse visual cortex. We find spontaneous activity correlated among networks of layer 5 pyramidal cells. Synchronous ensembles occupy overlapping territories, often share neurons, and are repeatedly activated. Sets of neurons are also sequentially activated numerous times. Network synchronization and sequential correlations are blocked by glutamatergic antagonists, even though spontaneous firing persists in many "autonomously active" neurons. This autonomous activity is periodic and depends on hyperpolarization-activated cationic (H) and persistent sodium (Na-p) currents. We conclude that the isolated neocortical microcircuit generates spontaneous activity, mediated by a combination of intrinsic and circuit mechanisms, and that this activity can be temporally precise.