Irradiation with low-dose gamma ray enhances tolerance to heat stress in Arabidopsis seedlings.
Irradiation with low-dose gamma ray enhances tolerance to heat stress in Arabidopsis seedlings.
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DOI：
10.1016/j.ecoenv.2016.02.025
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发表时间：
2016-06
影响因子：
6.8
通讯作者：
Liang Zhang;F. Zheng;Wencai Qi;Tianqi Wang;Lingyu Ma;Z. Qiu;Jingyuan Li
中科院分区：
文献类型：
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作者：
Liang Zhang;F. Zheng;Wencai Qi;Tianqi Wang;Lingyu Ma;Z. Qiu;Jingyuan Li
低剂量γ射线辐照可以刺激植物对环境胁迫的耐受性。然而，有关低剂量γ辐射诱导耐受性增强的机制的知识远未完全理解。在这项研究中，调查的生理和分子机制的热胁迫缓解低剂量γ射线照射，拟南芥种子暴露于一系列的剂量进行热处理之前。结果表明，50-戈伊γ射线照射能最大限度地促进幼苗对热胁迫的反应。热胁迫下，经50~戈伊剂量辐照的幼苗超氧阴离子自由基产生速率、过氧化氢和丙二醛含量均显著低于对照。与对照相比，γ射线处理后幼苗体内抗氧化酶活性、谷胱甘肽（GSH）含量和脯氨酸水平均显著提高。此外，选定的基因的转录表达分析表明，一些组件相关的耐热性刺激低剂量γ辐射热休克下。结果表明，低剂量γ射线辐照可以调节拟南芥幼苗的生理反应以及与耐热性相关的基因表达，从而减轻逆境胁迫对拟南芥幼苗的伤害。
Gamma irradiation at low doses can stimulate the tolerance to environmental stress in plants. However, the knowledge regarding the mechanisms underlying the enhanced tolerance induced by low-dose gamma irradiation is far from fully understood. In this study, to investigate the physiological and molecular mechanisms of heat stress alleviated by low-dose gamma irradiation, the Arabidopsis seeds were exposed to a range of doses before subjected to heat treatment. Our results showed that 50-Gy gamma irradiation maximally promoted seedling growth in response to heat stress. The production rate of superoxide radical and contents of hydrogen peroxide and malondialdehyde in the seedlings irradiated with 50-Gy dose under heat stress were significantly lower than those of controls. The activities of antioxidant enzymes, glutathione (GSH) content and proline level in the gamma-irradiated seedlings were significantly increased compared with the controls. Furthermore, transcriptional expression analysis of selected genes revealed that some components related to heat tolerance were stimulated by low-dose gamma irradiation under heat shock. Our results suggest that low-dose gamma irradiation can modulate the physiological responses as well as gene expression related to heat tolerance, thus alleviating the stress damage inArabidopsisseedlings.