Isolation and Analysis of Bacteria with Antimicrobial Activities from the Marine Sponge Haliclona simulans Collected from Irish Waters
Isolation and Analysis of Bacteria with Antimicrobial Activities from the Marine Sponge Haliclona simulans Collected from Irish Waters
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DOI：
10.1007/s10126-008-9154-1
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发表时间：
2009-06-01
影响因子：
3
通讯作者：
Dobson, Alan D. W.
中科院分区：
文献类型：
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作者：
Kennedy, Jonathan;Baker, Paul;Dobson, Alan D. W.
从爱尔兰沿海水域收集了海绵体模拟海绵体的样本，并从这些样本中分离出细菌。对培养菌株进行系统发育分析，发现有4个不同的细菌门；细菌门，放线菌门，变形菌门和厚壁菌门。对海绵细菌分离株进行了抗菌物质生产检测，并证明了对革兰氏阳性和革兰氏阴性细菌和真菌的生物活性，50%的分离株对至少一种测试菌株具有抗菌活性。进一步的实验表明，其抑菌活性扩展到铜绿假单胞菌、艰难梭菌、耐多药金黄色葡萄球菌和致病性酵母菌。放线菌是数字上最丰富的抗菌活性生产者，尽管从芽孢杆菌和假弧菌分离物中也注意到活性。对编码多酮合成酶和非核糖体肽合成酶基因的潜在抗生素存在的调查也表明，这些次生代谢物的生物合成基因存在于大多数细菌门中，但在放线菌门和变形菌门中尤其普遍。本研究表明，海绵拟合菌的可培养部分是多种多样的，似乎具有很大的潜力，作为发现新的医学相关生物活性剂的来源。
Samples of the marine sponge Haliclona simulans were collected from Irish coastal waters, and bacteria were isolated from these samples. Phylogenetic analyses of the cultured isolates showed that four different bacterial phyla were represented; Bacteriodetes, Actinobacteria, Proteobacteria, and Firmicutes. The sponge bacterial isolates were assayed for the production of antimicrobial substances, and biological activities against Gram-positive and Gram-negative bacteria and fungi were demonstrated, with 50% of isolates showing antimicrobial activity against at least one of the test strains. Further testing showed that the antimicrobial activities extended to the important pathogens Pseudomonas aeruginosa, Clostridium difficile, multi-drug-resistant Staphylococcus aureus, and pathogenic yeast strains. The Actinomycetes were numerically the most abundant producers of antimicrobial activities, although activities were also noted from Bacilli and Pseudovibrio isolates. Surveys for the presence of potential antibiotic encoding polyketide synthase and nonribosomal peptide synthetase genes also revealed that genes for the biosynthesis of these secondary metabolites were present in most bacterial phyla but were particularly prevalent among the Actinobacteria and Proteobacteria. This study demonstrates that the culturable fraction of bacteria from the sponge H. simulans is diverse and appears to possess much potential as a source for the discovery of new medically relevant biological active agents.