Multiplex bioimaging of single-cell spatial profiles for precision cancer diagnostics and therapeutics
Multiplex bioimaging of single-cell spatial profiles for precision cancer diagnostics and therapeutics
复制标题
单细胞空间轮廓的多重生物成像用于精确癌症诊断和治疗
DOI：
10.1038/s41698-020-0114-1
复制
发表时间：
2020-05-01
影响因子：
7.9
通讯作者：
Coskun, Ahmet F.
中科院分区：
文献类型：
--
作者：
Allam, Mayar;Cai, Shuangyi;Coskun, Ahmet F.
癌症在不同的患者中表现出功能和结构的多样性。在这个肿块中，正常和恶性细胞创造了患者之间异质的肿瘤微环境。原发性肿瘤的残留物以细胞簇和单细胞的形式泄漏到血流中，为疾病进展和治疗反应提供线索。这些层次化微环境的复杂性需要阐明。尽管肿瘤包含丰富的细胞类型，但标准的临床技术仍然是限于单一标记物的组织学。多重成像技术开辟了病理学的新方向。人类癌症的空间分辨蛋白质组学、基因组学和代谢谱现在可以在单细胞水平上进行。这个观点讨论了空间生物成像方法来破译固体和液体活检微环境的级联。一个独特的自上而下和自下而上的分析方法的合成。空间多组学图谱可以通过人工智能定制为精确的肿瘤学。数据驱动的患者分析可实现个性化医疗及其他。
Cancers exhibit functional and structural diversity in distinct patients. In this mass, normal and malignant cells create tumor microenvironment that is heterogeneous among patients. A residue from primary tumors leaks into the bloodstream as cell clusters and single cells, providing clues about disease progression and therapeutic response. The complexity of these hierarchical microenvironments needs to be elucidated. Although tumors comprise ample cell types, the standard clinical technique is still the histology that is limited to a single marker. Multiplexed imaging technologies open new directions in pathology. Spatially resolved proteomic, genomic, and metabolic profiles of human cancers are now possible at the single-cell level. This perspective discusses spatial bioimaging methods to decipher the cascade of microenvironments in solid and liquid biopsies. A unique synthesis of top-down and bottom-up analysis methods is presented. Spatial multi-omics profiles can be tailored to precision oncology through artificial intelligence. Data-driven patient profiling enables personalized medicine and beyond.