Accumulation of advanced glycation endproducts reduces chondrocyte-mediated extracellular matrix turnover in human articular cartilage
Accumulation of advanced glycation endproducts reduces chondrocyte-mediated extracellular matrix turnover in human articular cartilage
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DOI：
10.1053/joca.2001.0469
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发表时间：
2001-11-01
影响因子：
7
通讯作者：
Lafeber, FPJG
中科院分区：
文献类型：
--
作者：
DeGroot, J;Verzijl, N;Lafeber, FPJG
目的：骨性关节炎(OAS)的患病率随着年龄的增长而增加，并与晚期糖基化终产物(AGEs)在关节软骨中的积聚相一致，提示糖基化产物的积聚可能参与了OA的发生发展。本研究旨在探讨AGEs积聚对人关节软骨细胞外基质周转的影响。设计：测定19~82岁(N=30，培养4天)人关节软骨细胞介导的软骨降解(GAG释放，比色法)。此外，为了在体外模拟与年龄相关的AGE水平的增加，在没有或存在葡萄糖、核糖或苏糖的情况下培养软骨。结果：软骨细胞介导的关节软骨降解(即释放GAG)随年龄增长而减少(r=-0.43，P
Objective: The prevalence of osteoarthritis (OAs) increases with age and coincides with the accumulation of advanced glycation endproducts (AGEs) in articular cartilage, suggesting that accumulation of glycation products may be involved in the development of OA. This study was designed to examine the effects of accumulation of AGEs on the turnover of the extracellular matrix of human articular cartilage.Design: Chondrocyte mediated cartilage degradation (GAG release, colorimetric) was measured in human articular cartilage of donors aged 19-82 years (N=30, 4-day culture). In addition, to mimic the age-related increase in AGE levels in vitro, cartilage was cultured in the absence or presence of glucose, ribose or threose. Cartilage degradation and proteoglycan synthesis ((SO42-)-S-35 incorporation) were measured and related to the degree of cartilage AGE levels (fluorescence at 360/460 nm).Results: Chondrocyte-mediated degradation of articular cartilage (i.e. GAG release) decreased with increasing age of the cartilage donor (r= -0.43, P