Genome-wide identification and expression profiling of the cystatin gene family in apple (Malus × domestica Borkh.).
Genome-wide identification and expression profiling of the cystatin gene family in apple (Malus × domestica Borkh.).
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DOI：
10.1016/j.plaphy.2014.03.011
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发表时间：
2014-06
期刊：
影响因子：
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通讯作者：
Yanxiao Tan;Suncai Wang;D. Liang;Mingjun Li;F. Ma
中科院分区：
文献类型：
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作者：
Yanxiao Tan;Suncai Wang;D. Liang;Mingjun Li;F. Ma
半胱氨酸酶抑制剂或植物半胱氨酸蛋白酶抑制剂(PhyCys)是一类植物特异性半胱氨酸蛋白酶抑制剂。这些抑制物被认为参与了几个内源过程的调节，以及对生物或非生物胁迫的防御。然而，关于这个家庭的信息在苹果上是有限的。我们在整个苹果基因组中确定了26个PhyCys基因。它们被分成三个不同的组，分布在几条染色体上。它们的所有推测蛋白都含有一个或两个典型的半胱氨酸氨基转移酶结构域，这些结构域具有PhyCys的特征基序。选择的8个基因在不同组织中显示了差异表达模式。此外，在叶片成熟、衰老或对一个或多个非生物胁迫处理的响应中，它们的转录水平也显著上调。我们的结果表明，该家族的成员可能在苹果的组织发育、叶片衰老和对不利环境的适应中发挥作用。
Cystatins or phytocystatins (PhyCys) comprise a family of plant-specific inhibitors of cysteine proteinases. Such inhibitors are thought to be involved in the regulation of several endogenous processes as well as defense against biotic or abiotic stresses. However, information about this family is limited in apple. We identified 26 PhyCys genes within the entire apple genome. They were clustered into three distinct groups distributed across several chromosomes. All of their putative proteins contained one or two typical cystatin domains, which shared the characteristic motifs of PhyCys. Eight selected genes displayed differential expression patterns in various tissues. Moreover, their transcript levels were also up-regulated significantly in leaves during maturation, senescence or in response to treatment with one or more abiotic stresses. Our results indicated that members of this family may function in tissue development, leaf senescence, and adaptation to adverse environments in apple.