Development of Double Eastern Blotting for Major Licorice Components, Glycyrrhizin and Liquiritin for Chemical Quality Control of Licorice Using anti-Glycyrrhizin and anti-Liquiritin Monoclonal Antibodies
Development of Double Eastern Blotting for Major Licorice Components, Glycyrrhizin and Liquiritin for Chemical Quality Control of Licorice Using anti-Glycyrrhizin and anti-Liquiritin Monoclonal Antibodies
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DOI：
10.1021/acs.jafc.5b04732
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发表时间：
2016-02-10
影响因子：
6.1
通讯作者：
Shoyama, Yukihiro
中科院分区：
文献类型：
--
作者：
Fujii, Shunsuke;Morinaga, Osamu;Shoyama, Yukihiro
许可证在世界各地的各种食品工业中用于调味剂、调味品、饮料和功能食品。甘草甜素（GL）和甘草苷（Liq）是甘草的主要质量控制化学标志物，具有多功能生物活性。甘草的化学质量控制是重要的，因为其成分的变化取决于环境因素（气候，土壤条件和水分亏缺）和物种之间的差异。为了更方便、快速和特异地对GL和Liq进行质量控制分析，分别开发了使用抗GL和抗Liq单克隆抗体的双重Eastern印迹法。此外，双重东部印迹法应用于调查GL和Liq的免疫组织化学分布在根，新鲜甘草，然后澄清的定位，这两个组件的视觉。GL和Liq的这种双重蛋白质印迹技术可以作为一种强有力的方法来直观地确定甘草的化学质量。
Licorice is utilized in various food industries around the world for seasoning agents, confectioneries, drinks, and fUnctional foods. Glycyrrhizin (GL) and liquiritin (Liq) are major quality control chemical markers of licorice that have multifunctional bioactivities. Chemical quality control of licorice is important because its component profiles change depending environmental factors (climate, soil condition, and water deficit) and differences between species. Double eastern blotting using anti-GL and anti-Liq monoclonal antibodies was developed for more convenient, rapid, and specific quality control analysis of GL and Liq, respectively. Moreover, double eastern blotting was applied to investigate the immunohistochemical distributions of GL and Liq in the root, of fresh licorice; the localization of both components was then clarified visually. This double eastern blotting technique for GL and Liq may serve as a powerful approach for visually determining the chemical quality of licorice.