Distinct microbial populations exist in the mucosa-associated microbiota of sub-groups of irritable bowel syndrome
Distinct microbial populations exist in the mucosa-associated microbiota of sub-groups of irritable bowel syndrome
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DOI：
10.1111/j.1365-2982.2011.01803.x
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发表时间：
2012-01-01
影响因子：
3.5
通讯作者：
Sanderson, J. D.
中科院分区：
文献类型：
--
作者：
Parkes, G. C.;Rayment, N. B.;Sanderson, J. D.
背景越来越多的证据支持胃肠道菌群在肠易激综合征（IBS）病因学中的作用。鉴于IBS的炎症组分的证据，粘膜相关的微生物群可能在其发病机制中起关键作用。目的是使用荧光原位杂交比较腹泻型IBS（IBS-D）、便秘型IBS（IBS-C）患者和对照组之间的粘膜相关微生物群，并将特定细菌群与个体IBS症状相关联。方法47例IBS患者（IBS-D型27例，IBS-C型20例）和26例健康对照者作为研究对象。在结肠镜检查时进行快速冷冻直肠活检，并通过将冷冻切片与细菌群特异性寡核苷酸探针杂交进行细菌定量。关键结果：IBS患者每mm直肠上皮中的总粘膜相关细菌数量显著高于对照组[中位数218（IQR - 209）vs 128（121）P = 0.007]，主要由肠易激综合征类杆菌[69（67）vs 14（41）P = 0.001]和直肠真杆菌-球状梭菌[52（58）vs 25（35）P = 0.001]组成。0.03]。对IBS亚组的分析表明，IBS-D组中的双歧杆菌低于IBS-C组和对照组[24（32）vs 54（88）vs 32（35）P = 0.011]。最后，在IBS患者中，每天的最大排便次数与粘膜相关双歧杆菌（P < 0.001）和乳酸杆菌（P = 0.002）的数量呈负相关。IBS患者的粘膜相关微生物群与健康对照组显著不同，IBS-D患者的类杆菌和梭菌增加，生物多样性细菌减少。
Background There is increasing evidence to support a role for the gastrointestinal microbiota in the etiology of irritable bowel syndrome (IBS). Given the evidence of an inflammatory component to IBS, the mucosa-associated microbiota potentially play a key role in its pathogenesis. The objectives were to compare the mucosa-associated microbiota between patients with diarrhea predominant IBS (IBS-D), constipation predominant IBS (IBS-C) and controls using fluorescent in situ hybridization and to correlate specific bacteria groups with individual IBS symptoms. Methods Forty-seven patients with IBS (27 IBS-D and 20 IBS-C) and 26 healthy controls were recruited to the study. Snap-frozen rectal biopsies were taken at colonoscopy and bacterial quantification performed by hybridizing frozen sections with bacterial-group specific oligonucleotide probes. Key Results Patients with IBS had significantly greater numbers of total mucosa-associated bacteria per mm of rectal epithelium than controls [median 218 (IQR - 209) vs 128 (121) P = 0.007], and this was chiefly comprised of bacteroides IBS [69 (67) vs 14 (41) P = 0.001] and Eubacterium rectale-Clostridium coccoides [52 (58) vs 25 (35) P = 0.03]. Analysis of IBS sub-groups demonstrated that bifidobacteria were lower in the IBS-D group than in the IBS-C group and controls [24 (32) vs 54 (88) vs 32 (35) P = 0.011]. Finally, amongst patients with IBS, the maximum number of stools per day negatively correlated with the number of mucosa-associated bifidobacteria (P < 0.001) and lactobacilli (P = 0.002). Conclusions & Inferences The mucosa-associated microbiota in patients with IBS is significantly different from healthy controls with increases in bacteroides and clostridia and a reduction in bifidobacteria in patients with IBS-D.