Green synthesis of poly-L-lysine-coated sericin nanoparticles and their molecular size-dependent antibacterial activity
Green synthesis of poly-L-lysine-coated sericin nanoparticles and their molecular size-dependent antibacterial activity
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DOI：
10.1016/j.colsurfb.2020.110822
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发表时间：
2020-04-01
影响因子：
5.8
通讯作者：
Ghosh, Ananta Kumar
中科院分区：
文献类型：
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作者：
Dutta, Soumita;Chowdhury, Trinath;Ghosh, Ananta Kumar
研究纳米粒子(NPs)的分子大小和界面电位对细菌的抗菌作用是确保NPs在任何生物系统中安全使用的重要目标。本研究将蚕茧中的粗丝丝胶蛋白分成组分-1(50-300 kDa)、组分-2(30-50 kDa)和组分-3(10-30 kDa)，分别用于制备粗丝胶纳米粒(CRSNPs)和比表面负势纳米粒：n-SNP1、n-SNP2和n-SNP3。用多聚L赖氨酸包覆SNPs，使其表面电位正化，并通过UV-Vis光谱、傅立叶变换红外光谱、Zeta粒度和Zeta电位测量对其进行了表征。所有SNPs的形状和大小均为球形，其中n-SNPs为110~165 nm，p-SNPs为66~85 nm，SNP2为33~49 nm，SNP3为14~24 nm。用不同浓度(50、100、200微克/毫升)的SNPs对金黄色葡萄球菌和大肠埃希氏菌进行抗菌活性测定，发现p-SNPs的抗菌活性显著高于n-SNPs。其中，SNP2的抗菌活性最强，其次是SNP3、SNP1和CRSNPs。对人体细胞无毒的p-SNP2(50微克/毫升)处理细菌后，观察到相对较高的活性氧(ROS)生成量。Fe-SEM分析表明，p-SNPs处理后细菌细胞膜的破坏比n-SNPs处理后更严重。所有这些数据表明，SNPs的分子尺寸和界面电位促进了ROS的产生，从而发挥了其抗菌活性。
Investigating the role of molecular size and interfacial potential dependent antimicrobial propensity of nano-particles (NPs) against bacteria is the important goal for secure usage of NPs to any living systems. In this study, crude silk sericin protein of Antheraea mylitta cocoon was fractionated into three different molecular size-ranges fractions such as fraction-1 (50-300 kDa), fraction-2 (30-50 kDa) and fraction-3 (10-30 kDa), and used to prepare crude sericin nanoparticles (CRSNPs), as well as fraction specific negative surface potential nanoparticles : n-SNP1, n-SNP2 and n-SNP3, respectively. SNPs were coated with poly-L-lysine to make the surface potential positive (p-SNPs) and confirmed through UV-vis spectroscopy, FTIR, zeta sizer and zeta potential measurement. The shape and sizes of all SNPs were determined by electron microscopy and found spherical in shape having diameter ranging from 110-165 nm (CRSNPs), 66-85 nm (SNP1), 33-49 nm (SNP2) and 14-24 nm (SNP3) for n-SNPs and p-SNPs, respectively. Evaluation of antibacterial activity using different concentrations (50, 100, 200 mu g/mL) of all these SNPs showed significantly more activity of p-SNPs than n-SNPs against Staphylococcus aureus and Escherichia coli. Among these, SNP2 showed the strongest antibacterial activity followed by SNP3, SNP1 and CRSNPs. Relatively higher amounts of reactive oxygen species (ROS) generation were observed after treatment of bacteria with p-SNP2 (50 mu g/mL) which is non-toxic to human cells. FE-SEM analysis showed more disruption of bacterial cell membrane after treatment with p-SNPs than n-SNPs. All these data suggested that molecular size and interfacial potential of SNPs enhance ROS generation to exert their antibacterial activity.