Cofilin promotes actin polymerization and defines the direction of cell motility
Cofilin promotes actin polymerization and defines the direction of cell motility
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DOI：
10.1126/science.1094561
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发表时间：
2004-04-30
期刊：
影响因子：
56.9
通讯作者：
Condeelis, JS
中科院分区：
文献类型：
--
作者：
Ghosh, M;Song, XY;Condeelis, JS
一个通用的笼的方法，由磷酸化调节的蛋白质被用来研究在体内的生物化学作用的cofilin和随后的细胞反应。通过急性和局部激活的化学工程，光敏感的phosphocofilin模拟，我们证明，cofilin聚合肌动蛋白，产生突起，并确定细胞迁移的方向。我们提出了一个cofilin的作用，这是不同于它的作用作为一个肌动蛋白解聚因子。
A general caging method for proteins that are regulated by phosphorylation was used to study the in vivo biochemical action of cofilin and the subsequent cellular response. By acute and local activation of a chemically engineered, light-sensitive phosphocofilin mimic, we demonstrate that cofilin polymerizes actin, generates protrusions, and determines the direction of cell migration. We propose a role for cofilin that is distinct from its role as an actin-depolymerizing factor.