Genome Wide Identification and Characterization of Apple bHLH Transcription Factors and Expression Analysis in Response to Drought and Salt Stress

Genome Wide Identification and Characterization of Apple bHLH Transcription Factors and Expression Analysis in Response to Drought and Salt Stress
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苹果 bHLH 转录因子的全基因组鉴定和表征以及响应干旱和盐胁迫的表达分析

DOI:
10.3389/fpls.2017.00480
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发表时间:
2017-04-11
影响因子:
5.6
通讯作者:
Ma, Fengwang
Ma, Fengwang
中科院分区:
生物学2区
文献类型:
--
作者:
Mao, Ke;Dong, Qinglong;Ma, Fengwang

文献摘要

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bHLH(basic helix-loop-helix)转录因子家族是植物中第二大转录因子家族。它存在于所有三个真核生物界,并在调节生长和发育中发挥重要作用。然而,苹果公司此前从未对家族成员进行过研究。本研究从金冠苹果中鉴定了188个MdbHLH蛋白,可分为18类。我们还研究了这些MdbHLH的基因结构和12个保守基序。再加上表达分析和蛋白质相互作用网络预测,我们确定了几个基因,可能是负责非生物胁迫反应。本研究为苹果中此类蛋白的后续研究提供了见解和丰富的资源。
The bHLH (basic helix-loop-helix) transcription factor family is the second largest in plants. It occurs in all three eukaryotic kingdoms, and plays important roles in regulating growth and development. However, family members have not previously been studied in apple. Here, we identified 188 MdbHLH proteins in apple “Golden Delicious” (Malus × domestica Borkh.), which could be classified into 18 groups. We also investigated the gene structures and 12 conserved motifs in these MdbHLHs. Coupled with expression analysis and protein interaction network prediction, we identified several genes that might be responsible for abiotic stress responses. This study provides insight and rich resources for subsequent investigations of such proteins in apple.