Evaluation of automated sample preparation, retention time locked gas chromatography-mass spectrometry and data analysis methods for the metabolomic study of Arabidopsis species
Evaluation of automated sample preparation, retention time locked gas chromatography-mass spectrometry and data analysis methods for the metabolomic study of Arabidopsis species
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DOI：
10.1016/j.chroma.2011.01.024
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发表时间：
2011-05-27
影响因子：
4.1
通讯作者：
Sandra, Pat
中科院分区：
文献类型：
--
作者：
Gu, Qun;David, Frank;Sandra, Pat
本文对自动样品制备、保留时间锁定气相色谱-质谱（GC-MS）和数据分析方法进行了评价。拟南芥（Arabidopsis thaliana）是植物科学和遗传学中常用的模式生物，采用了一种小型化和自动化衍生化方法，采用顺序氧化和硅基化方法对4种类型（2种类型x 2种年龄）的极性提取物进行了分析。衍生化过程的自动化提供了极好的重复性，样品制备和分析之间的时间短而恒定，减少了伪影的形成。使用保留时间锁定（RTL）气相色谱-质谱法，获得可重复的保留时间和GC-MS谱。数据分析采用了两种方法。比较了XCMS采用主成分分析（方法1）和AMDIS反褶积结合市售程序（Mass Profiler Professional）采用主成分分析（方法2）。在不同类型中检测到上调或下调的几个特征。(C) 2011 Elsevier B.V.版权所有
In this paper, automated sample preparation, retention time locked gas chromatography-mass spectrometry (GC-MS) and data analysis methods for the metabolomics study were evaluated. A miniaturized and automated derivatisation method using sequential oximation and silylation was applied to a polar extract of 4 types (2 types x 2 ages) of Arabidopsis thaliana, a popular model organism often used in plant sciences and genetics. Automation of the derivatisation process offers excellent repeatability, and the time between sample preparation and analysis was short and constant, reducing artifact formation. Retention time locked (RTL) gas chromatography-mass spectrometry was used, resulting in reproducible retention times and GC-MS profiles. Two approaches were used for data analysis. XCMS followed by principal component analysis (approach 1) and AMDIS deconvolution combined with a commercially available program (Mass Profiler Professional) followed by principal component analysis (approach 2) were compared. Several features that were up- or down-regulated in the different types were detected. (C) 2011 Elsevier B.V. All rights reserved.