Synthesis of immunoglobulins against Haemophilus parainfluenzae by tonsillar lymphocytes from patients with IgA nephropathy.
Synthesis of immunoglobulins against Haemophilus parainfluenzae by tonsillar lymphocytes from patients with IgA nephropathy.
复制标题
IgA 肾病患者扁桃体淋巴细胞合成抗副流感嗜血杆菌免疫球蛋白。
DOI：
10.1093/ndt/15.5.619
复制
发表时间：
2000
期刊：
影响因子：
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通讯作者：
F. Gejyo
中科院分区：
文献类型：
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作者：
S. Suzuki;S. Fujieda;H. Sunaga;C. Yamamoto;H. Kimura;F. Gejyo
背景
我们以前证明肾小球沉积的副流感嗜血杆菌（HP）抗原和伊加肾病（IgAN）患者的伊加抗体对HP抗原的存在。在这份报告中，我们研究了免疫球蛋白的合成对HP抗原在扁桃体淋巴细胞与IgA肾病患者。
方法
我们使用的扁桃体淋巴细胞分离自15例IgAN和16例慢性扁桃体炎，但没有肾脏疾病的腭扁桃体。我们检查淋巴细胞增殖和伊加，IgG和IgM抗体对HP抗原的生产，通过测量胸苷的摄取和这些抗体在培养上清液中的浓度淋巴细胞孵育后，HP抗原通过ELISA。
结果
来自IgAN患者的淋巴细胞在暴露于HP抗原时显示出显著更高的刺激指数（SI）（暴露于HP的扁桃体淋巴细胞中的胸苷掺入（c. p.m.）/未刺激扁桃体淋巴细胞中的胸苷掺入（c. p.m.））与对照组相比（P=0. 0015）。IgAN患者淋巴细胞的伊加SI（HP刺激淋巴细胞上清液中抗HP抗原的IgA浓度/未刺激扁桃体淋巴细胞上清液中抗HP抗原的IgA浓度）也显著高于对照组（P=0.0004）。我们发现，HP刺激后，伊加和IgG抗体浓度之间，伊加和IgM抗体之间，IgG和IgM抗体之间对HP抗原呈正相关。
结论
我们的结果表明，HP抗原刺激IgAN患者的扁桃体T和B淋巴细胞，并且这些患者具有针对HP抗原的淋巴细胞多克隆活化，抗体产生的同种型从IgM转变为伊加。
BACKGROUND
We previously demonstrated glomerular deposition of Haemophilus parainfluenzae (HP) antigens and the presence of IgA antibody against HP antigens in patients with IgA nephropathy (IgAN). In this report we examine the synthesis of immunoglobulins against HP antigens in tonsillar lymphocytes from patients with IgAN.
METHODS
We used tonsillar lymphocytes isolated from the palatine tonsils of 15 patients with IgAN and 16 patients with chronic tonsillitis but without renal disease. We examined lymphocyte proliferation and production of IgA, IgG, and IgM antibodies against HP antigens by measuring thymidine uptake and concentrations of these antibodies in culture supernatants after lymphocyte incubation with HP antigens by ELISA.
RESULTS
Lymphocytes from patients with IgAN showed a significantly higher stimulation index (SI) on exposure to HP antigens (thymidine incorporation in tonsillar lymphocytes exposed to HP (c.p.m.)/ thymidine incorporation in unstimulated tonsillar lymphocytes (c.p.m.)) than did controls (P=0. 0015). Lymphocytes from patients with IgAN also showed a significantly higher IgA SI (concentrations of IgA against HP antigens in supernatants from HP-stimulated lymphocytes/IgA against HP antigens in supernatants from unstimulated tonsillar lymphocytes) than did controls (P=0.0004). We found positive correlations between concentrations of IgA and IgG antibodies, between IgA and IgM antibodies, and between IgG and IgM antibodies against HP antigens after HP stimulation.
CONCLUSIONS
Our results suggest that HP antigens stimulate tonsillar T and B lymphocytes in patients with IgAN and that these patients have polyclonal activation of lymphocytes against HP antigens, with isotype switching of antibody production from IgM to IgA.
Negoro N、Kanayama Y、Inariba H、Okamura M、Inoue T、Takeda T：“内皮素调节自发性高血压大鼠培养的主动脉平滑肌细胞中的 c-myc 原癌原表达”高血压。
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