TRANSFORMING GROWTH FACTORS(S) PRODUCTION ENABLES CELLS TO GROW IN THE ABSENCE OF SERUM - AN AUTOCRINE SYSTEM
TRANSFORMING GROWTH FACTORS(S) PRODUCTION ENABLES CELLS TO GROW IN THE ABSENCE OF SERUM - AN AUTOCRINE SYSTEM
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DOI：
10.1073/pnas.79.2.485
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发表时间：
1982-01-01
期刊：
影响因子：
--
通讯作者：
OZANNE, B
中科院分区：
文献类型：
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作者：
KAPLAN, PL;ANDERSON, M;OZANNE, B
Kirsten小鼠肉瘤病毒(KiMSV)转化的大鼠-1、正常大鼠肾脏(NRK)和BALB/c 3T3细胞能够在添加转铁蛋白和胰岛素但不含外源性丝裂原生长因子的无血清培养液中持续生长。由对保持转化的温度敏感的KiMSV突变体转化的细胞仅在允许的温度下在该介质中生长。在不允许的温度下，生长依赖于转化细胞的无血清条件培养液的存在。正常的大鼠-1细胞也依赖于转化细胞的因子在这种无血清/无有丝分裂原的培养液中生长。血清来源的生长因子、表皮生长因子和成纤维细胞生长因子对转化细胞没有影响，但表皮生长因子可以替代KiMSV转化细胞产生的转化生长因子来促进大鼠1细胞的生长。转化细胞在无血清培养基中以克隆密度生长依赖于从生长到高密度的相同细胞收集的条件培养液的存在。无血清/无丝裂原培养是KiMSV转化细胞的普遍特性，转化细胞在该培养基中的生长依赖于细胞所产生的生长因子的存在。KiMSV转化细胞的无血清生长似乎依赖于异位产生的生长因子。
Kirsten murine sarcoma virus (KiMSV)-transformed rat-1, normal rat kidney (NRK) and BALB/c 3T3 cells are capable of continual growth in a serum-free medium supplemented with transferrin and insulin but with no exogenous mitogenic growth factors. Cells transformed by a mutant of KiMSV that is temperature-sensitive for the maintenance of transformation grow in this medium at the permissive temperature only. At the nonpermissive temperature, growth is dependent upon the presence of serum-free conditioned medium from the transformed cells. Normal rat-1 cells are also dependent upon factors from the transformed cells for growth in this serum-free/mitogen-free medium. The serum-derived growth factors, epidermal growth factor and fibroblast growth factor have no effect on the transformed cells, although epidermal growth factor can replace transforming growth factors produced by KiMSV-transformed cells for the growth of rat-1 cells. Growth of the transformed cells in serum-free medium at clonal densities is dependent upon the presence of conditioned medium collected from the same cells grown to high densities. Growth in serum-serum-free/mitogen-free medium is a general property of KiMSV-transformed cells, and growth of the transformed cells in this medium is dependent upon the presence of growth factors known to be produced by the cells. Serum-free growth of KiMSV-transformed cells appears to be dependent upon ectopically-produced growth factors.