Activity Correlation Imaging: Visualizing Function and Structure of Neuronal Populations
Activity Correlation Imaging: Visualizing Function and Structure of Neuronal Populations
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DOI：
10.1016/j.bpj.2008.12.3962
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发表时间：
2009-05-06
影响因子：
3.4
通讯作者：
Schildt, Detlev
中科院分区：
文献类型：
--
作者：
Junek, Stephan;Chen, Tsai-Wen;Schildt, Detlev
在神经网络的分析中，将神经元的活动与其形态联系起来是一个重要但尚未解决的问题。在这里，我们引入活动相关成像来同时可视化神经元群体的活动和形态。为此，我们首先使用膜渗透性[Ca2+]指示剂（例如Fluo-4/AM）对网络神经元进行染色，并记录它们的活动。然后，我们利用记录的时间活动模式作为内在对比的手段来可视化单个神经元的树突形态。结果是神经网络的高对比度、多色可视化。以非洲爪蟾嗅球为例，我们展示了嗅球的二尖瓣/簇状细胞的活动以及它们向嗅肾小球的投射。这种方法，产生神经细胞群的功能和结构信息，将为神经网络的研究开辟前所未有的可能性。
For the analysis of neuronal networks it is an important yet unresolved task to relate the neurons' activities to their morphology. Here we introduce activity correlation imaging to simultaneously visualize the activity and morphology of populations of neurons. To this end we first stain the network's neurons using a membrane-permeable [Ca2+] indicator (e.g., Fluo-4/AM) and record their activities. We then exploit the recorded temporal activity patterns as a means of intrinsic contrast to visualize individual neurons' dendritic morphology. The result is a high-contrast, multicolor visualization of the neuronal network. Taking the Xenopus olfactory bulb as an example we show the activities of the mitral/tufted cells of the olfactory bulb as well as their projections into the olfactory glomeruli. This method, yielding both functional and structural information of neuronal populations, will open up unprecedented possibilities for the investigation of neuronal networks.