Identification of Two IgD+ B Cell Populations in Channel Catfish, Ictalurus punctatus
Identification of Two IgD+ B Cell Populations in Channel Catfish, Ictalurus punctatus
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DOI：
10.4049/jimmunol.1000631
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发表时间：
2010-10-01
影响因子：
4.4
通讯作者：
Wilson, Melanie
中科院分区：
文献类型：
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作者：
Edholm, Eva-Stina;Bengten, Eva;Wilson, Melanie
癍点叉尾（Ictalurus punctatus）表达两种IG同种型：IgM和IgD。虽然鲶鱼IgM已经在功能和结构水平上进行了广泛的研究，但对IgD的了解要少得多。在这项研究中，IgM（+）/IgD（+）和IgM（-）/IgD（+）鲶鱼B细胞群体通过使用抗IgM和抗IgD单克隆抗体进行鉴定。鲶鱼IgM（+）/IgD（+）B细胞较小且无颗粒。相比之下，IgM（-）/IgD（+）B细胞更大，并表现出浆母细胞形态。细胞分选、流式细胞术和RT-PCR的使用表明IgD（+）B细胞表达在个体之间存在差异。例如，有些鲶鱼
Channel catfish Ictalurus punctatus express two Ig isotypes: IgM and IgD. Although catfish IgM has been extensively studied at the functional and structural levels, much less is known about IgD. In this study, IgM(+)/IgD(+) and IgM(-)/IgD(+) catfish B cell populations were identified through the use of anti-IgM and anti-IgD mAbs. Catfish IgM(+)/IgD(+) B cells are small and agranular. In contrast, IgM(-)/IgD(+) B cells are larger and exhibit a plasmablast morphology. The use of cell sorting, flow cytometry, and RT-PCR demonstrated that IgD(+) B cell expression varies among individuals. For example, some catfish have