High-performance cation-exchange chromatography of proteins.
High-performance cation-exchange chromatography of proteins.
复制标题
蛋白质的高性能阳离子交换色谱。
DOI：
10.1016/0003-2697(83)90363-9
复制
发表时间：
1983
影响因子：
2.9
通讯作者：
Regnier,FE
中科院分区：
文献类型：
--
作者：
Gupta,S;Pfannkoch,E;Regnier,FE
在文献中报道的所有蛋白质中，约有三分之一的pI足够高，可以通过阳离子交换色谱分离。本文报道了新型高性能聚合物键合相阳离子交换柱的制备和应用。从一个非常稳定的，共价键合的聚酰胺涂层微粒二氧化硅开始，简单的衍生化产生一个通用的阳离子交换材料，用于传统上进行的经典羧甲基化的软凝胶支持分离。使用胰凝乳蛋白酶原、细胞色素c和溶菌酶作为标准品监测柱行为。的聚合物键合相是稳定的pH值为2.5，并表现出增强的蛋白质的选择性，由于轻微的疏水性的矩阵。几个分离的生物利益，证明这些小阳离子交换柱的现代生化分离的效用。
Approximately one-third of all proteins reported in the literature have a pI sufficiently high to be resolved by cation-exchange chromatography. This paper reports the preparation and use of new high-performance polymeric-bonded-phase cation-exchange columns. Starting from a very stable, covalently bonded polyamide coating on microparticulate silica, simple derivatization produces a versatile cation-exchange material useful for separations traditionally performed on classical carboxymethylated soft gel supports. Column behavior was monitored using chymotrypsinogen, cytochrome c, and lysozyme as standards. The polymeric bonded phase was stable to pH 2.5 and exhibits enhanced selectivity for proteins due to a slight hydrophobic character of the matrix. Several separations of biological interest that demonstrate the utility of these small cation-exchange columns for modern biochemical separations are shown.