Molecular identification of Palearctic members of Anopheles maculipennis in northern Iran
Molecular identification of Palearctic members of Anopheles maculipennis in northern Iran
复制标题
DOI：
10.1186/1475-2875-6-6
复制
发表时间：
2007-01-17
期刊：
影响因子：
3
通讯作者：
Zakeri, Sedigheh
中科院分区：
文献类型：
--
作者：
Djadid, Navid D.;Gholizadeh, Saber;Zakeri, Sedigheh
背景：马库利按蚊复合群的成员是欧洲和伊朗北部里海地区的有效疟疾媒介，自 1994 年以来，疟疾已重新传入该地区。当前的研究旨在提供有关物种组成状况的进一步证据，并更准确地识别伊朗北部马库利彭尼斯复合体的成员。方法：对核糖体 DNA 的第二个内部转录间隔区 (rDNA-ITS2) 进行测序伊朗东阿塞拜疆、阿尔代比勒、吉兰、马赞德兰和呼拉桑五个省采集的235份标本中的28份。结果：ITS2长度范围为283~302 bp，GC含量为49.33~54.76%。没有观察到种内变异。基于ITS2序列的系统发育树的构建表明，maculipennis复合体的六个伊朗成员可以很容易地分为三类：An. atroparvus - 按蚊组； An 的并系群。马库利彭尼斯，An.梅西埃，安.佩尔森斯;和安。 sacharovi为第三组。结论：检测到3种按蚊。 maculipennis 复合体包括 An.黑眼鱼，An.梅西和安.伊朗北部的新记录显示，拉布兰奇亚科细菌在某种程度上令人震惊。通过应用分子技术来正确识别物种复合体，通过寻找相关基因来了解杀虫剂抗药性的状态，可以更好地了解里海两岸的疟疾流行病学。
Background: Members of Anopheles maculipennis complex are effective malaria vectors in Europe and the Caspian Sea region in northern Iran, where malaria has been re-introduced since 1994. The current study has been designed in order to provide further evidence on the status of species composition and to identify more accurately the members of the maculipennis complex in northern Iran.Methods: The second internal transcribed spacer of ribosomal DNA (rDNA-ITS2) was sequenced in 28 out of 235 specimens that were collected in the five provinces of East Azerbayjan, Ardebil, Guilan, Mazandaran and Khorassan in Iran.Results: The length of the ITS2 ranged from 283 to 302 bp with a GC content of 49.33-54.76%. No intra-specific variations were observed. Construction of phylogenetic tree based on the ITS2 sequence revealed that the six Iranian members of the maculipennis complex could be easily clustered into three groups: the An. atroparvus - Anopheles labranchiae group; the paraphyletic group of An. maculipennis, An. messeae, An. persiensis; and An. sacharovi as the third group.Conclusion: Detection of three species of the An. maculipennis complex including An. atroparvus, An. messae and An. labranchiae, as shown as new records in northern Iran, is somehow alarming. A better understanding of the epidemiology of malaria on both sides of the Caspian Sea may be provided by applying the molecular techniques to the correct identification of species complexes, to the status of insecticide resistance by looking to related genes.