Differential expression of peroxisome proliferator-activated receptor delta at implantation sites and in decidual cells of rat uterus
Differential expression of peroxisome proliferator-activated receptor delta at implantation sites and in decidual cells of rat uterus
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DOI：
10.1530/reprod/125.6.817
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发表时间：
2003-06-01
期刊：
影响因子：
3.8
通讯作者：
Yang, ZM
中科院分区：
文献类型：
--
作者：
Ding, NZ;Ma, XH;Yang, ZM
本研究采用原位杂交和免疫组化的方法研究了过氧化物酶体增殖物激活受体（ppar - delta）基因在妊娠早期大鼠子宫中的表达和调控。PPARdelta mRNA在妊娠第1天高表达，从妊娠第2天开始逐渐下降，妊娠第5天无法检测到。然而，腺上皮的表达从妊娠第2天开始增加，并在妊娠第5天达到高表达。从第1天到第5天，管腔上皮和腺上皮未见PPARdelta免疫染色。妊娠第6天胚胎植入时，植入部位腔下间质中PPARdelta mRNA和免疫染色强烈。在第7天和第8天，在管腔附近的去个体化区域，PPARdelta mRNA的表达和免疫染色都很强。延迟着床期的腔下间质和腺上皮中PPARdelta表达较低。在雌激素处理终止延迟着床并开始胚胎着床后，腔下基质中PPARdelta mRNA和免疫染色均被强烈诱导。人工蜕膜下的蜕膜可见强烈的PPARdelta免疫染色，而未注射的对照角未见免疫染色。妊娠第6天植入囊胚周围腔下间质和妊娠第7、8天蜕膜细胞可见类视黄醇X受体（rxrα）免疫染色。综上所述，PPARdelta在大鼠子宫着床部位和蜕膜细胞中的高表达表明PPARdelta可能在着床和蜕膜过程中发挥重要作用。
The aim of this study was to examine the expression and regulation of peroxisome proliferator-activated receptor delta (PPARdelta) gene in rat uterus during early pregnancy by in situ hybridization and immunohistochemistry. PPARdelta mRNA expression in the luminal epithelium was high on day 1 of pregnancy, gradually declined from day 2 and was undetectable on day 5 of pregnancy. However, expression in the glandular epithelium began to increase from day 2 and was high on day 5 of pregnancy. There was no detectable PPARdelta immunostaining in the luminal and glandular epithelium from day 1 to day 5. On day 6 of pregnancy when embryos implanted, PPARdelta mRNA and immunostaining were intense in the subluminal stroma at implantation sites. On days 7 and 8, there was strong expression of both PPARdelta mRNA and intense immunostaining in the decidualized area near the lumen. There was low expression of PPARdelta in the subluminal stroma and glandular epithelium under delayed implantation. After delayed implantation was terminated by oestrogen treatment and embryo implantation was initiated, both PPARdelta mRNA and immunostaining were strongly induced in the subluminal stroma. Intense PPARdelta immunostaining was observed in the decidua under artificial decidualization, while no detectable immunostaining was seen in the uninjected control horn. Retinoid X receptor (RXRalpha) immunostaining was seen in the subluminal stroma surrounding the implanting blastocyst on day 6 and in the decidual cells on days 7 and 8 of pregnancy. In conclusion, the high PPARdelta expression at implantation sites and in the decidual cells in rat uterus indicates that PPARdelta may play an important role during implantation and decidualization.