The mitochondrial genome of Gyrodactylus derjavinoides (Platyhelminthes: Monogenea) -: A mitogenomic approach for Gyrodactylus species and strain identification
The mitochondrial genome of Gyrodactylus derjavinoides (Platyhelminthes: Monogenea) -: A mitogenomic approach for Gyrodactylus species and strain identification
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DOI：
10.1016/j.gene.2008.03.008
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发表时间：
2008-07-01
期刊：
影响因子：
3.5
通讯作者：
Littlewood, D. T. J.
中科院分区：
文献类型：
--
作者：
Huyse, Tine;Buchmann, Kurt;Littlewood, D. T. J.
系统分类学家和进化生物学家一直在寻找新的特征来源，以区分分类群，并估计分类群内部和之间的相互关系。整个线粒体基因组提供了丰富的数据，无论是在核苷酸和氨基酸水平。分子标记是特别有用的，当应用于小，形态上保守的类群，是许多单殖吸虫的鱼类外寄生虫的情况。三代虫属物种显示出相当程度的解剖保守性，使仅基于形态学的诊断变得复杂，并且有些是野生和养殖鱼类的重要害虫。在这里，我们测序了完整的线粒体基因组Gyrodactylus derjavinoides Malmberg，柯林斯，坎宁安和Behiar 2007，在斯堪的纳维亚半岛鲑鱼上最常见的gyrodactylid物种之一，并将其与最近发表的基因组Gyrodactylus salaris Malmberg，1957和Gyrodactylus thymalli Zitnan 1960进行比较。通过比较滑动窗口分析，我们确定了高序列变异性的区域，并设计了新的引物序列。总共开发了6对新引物，扩增了cox 1、cox 3、nad 1、nad 2、nad 4、nad 5和atp6的片段。它们共同扩增了捕获完整线粒体基因组中几乎一半核苷酸变异的区域。这些简并引物也应该适用于其他三代虫寄生鲑科动物。此外，我们开发了一种多重检测，同时扩增四个片段在一个单一的PCR反应。除了诊断价值外，这些片段还可用于研究三代虫的传播动力学，为更好地了解这些重要鱼类病原体的传播和流行病学提供重要信息。(c)2008 Elsevier B.V.保留所有权利。
Systematists and evolutionary biologists are constantly on the lookout for new sources of characters to discriminate amongst taxa and estimate interrelationships within and between taxa. Entire mitochondrial genomes provide a wealth of data, both at the nucleotide and amino acid level. Molecular markers are of particular utility when applied to small, morphologically conserved taxa, as is the case for many monogenean ectoparasites of fish. Gyrodactylus species display a considerable degree of anatomical conservatism, complicating diagnostics based solely on morphology, and some are significant pests of wild and cultured fish. Here we sequenced the complete mitochondrial genome of Gyrodactylus derjavinoides Malmberg, Collins, Cunningham & Behiar 2007, one of the most frequently found gyrodactylid species on salmonids in Scandinavia, and compared it with the recently published genomes of Gyrodactylus salaris Malmberg, 1957 and Gyrodactylus thymalli Zitnan 1960. Through comparative sliding window analysis we identified regions of high sequence variability and designed new primer sequences. In total, 6 new primer pairs have been developed, amplifying fragments of cox1, cox3, nad1, nad2, nad4, nad5 and atp6. Together, they amplify regions capturing almost half the nucleotide variability present in the complete mitochondrial genome. These degenerate primers should also work for other Gyrodactylus species parasitizing salmonids. In addition, we developed a multiplex assay that simultaneously amplifies four fragments in a single PCR reaction. Besides the diagnostic value, these fragments can be used for studying the transmission dynamics of Gyrodactylus, providing crucial information for an improved understanding of the spread and epidemiology of these important fish pathogens. (c) 2008 Elsevier B.V. All rights reserved.