TNF-α-308G>A and IL-6-174G>C polymorphisms in Tunisian patients with coronary artery disease
TNF-α-308G>A and IL-6-174G>C polymorphisms in Tunisian patients with coronary artery disease
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DOI：
10.1016/j.clinbiochem.2010.05.005
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发表时间：
2010-09-01
影响因子：
2.8
通讯作者：
Mahjoub, Touhami
中科院分区：
文献类型：
--
作者：
Ghazouani, Lakhdar;Khalifa, Sonia Ben Hadj;Mahjoub, Touhami
目的：我们的目的是评估肿瘤坏死因子(TNF)- α -308G>A和白细胞介素(IL)-6 -174G>C基因启动子变异对突尼斯人冠状动脉疾病（CAD）存在的贡献。设计和方法：研究对象包括418名经血管造影证实的CAD患者和406名年龄、性别和种族匹配的对照组。采用聚合酶链反应（PCR）和限制性片段长度多态性（RFLP）分析进行基因分型。结果：冠心病患者与对照组之间tnf - α -308A （19.6% vs. 19.0%, P = 0.73）和IL-6 -174C （15.6% vs. 14.3%, P = 0.47）启动子多态性的等位基因分布无显著差异。此外，单位点分析显示两个研究组之间基因型频率无差异，两种基因型的联合分布在对照组和CAD患者之间无显著差异（P < 0.05）。结论：在突尼斯人中，tnf - α -308G>A和IL-6 -174G>C启动子多态性与CAD没有等位基因或基因型关联，从而证实了这两种基因变异对CAD存在的种族选择性贡献。(C) 2010加拿大临床化学学会。Elsevier Inc.出版。版权所有。
Objective: Our aim was to evaluate the contribution of tumor necrosis factor (TNF)-alpha -308G>A and interleukin (IL)-6 -174G>C gene promoter variants to the presence of coronary artery disease (CAD) in Tunisians.Design and methods: Study subjects comprised 418 angiographically proven CAD patients and 406 age-, gender-, and ethnic origin-matched controls. Genotyping was performed using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis.Results: There were no significant differences in the allelic distribution of TNF-alpha -308A (19.6% vs. 19.0%, P = 0.73), and IL-6 -174C (15.6% vs. 14.3%, P = 0.47) promoter polymorphisms between CAD patients and control subjects, respectively. In addition, single locus analysis revealed no differences in genotype frequencies between the two study groups, and the combined distribution of both genotypes did not differ significantly between controls and CAD patients (P > 0.05).Conclusion: There is no allelic or genotypic association of TNF-alpha -308G>A and IL-6 -174G>C promoter polymorphisms with CAD in Tunisians, thereby confirming an ethnic-selective contribution of both gene variants to CAD presence. (C) 2010 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.