Characterization of Dof family in Pyrus bretschneideri and role of PbDof9.2 in flowering time regulation
Characterization of Dof family in Pyrus bretschneideri and role of PbDof9.2 in flowering time regulation
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Pyrus bretschneideri 中 Dof 家族的特征及 PbDof9.2 在开花时间调控中的作用
DOI：
10.1016/j.ygeno.2019.05.005
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发表时间：
2020-01-01
期刊：
影响因子：
4.4
通讯作者：
Wang, Peng
中科院分区：
文献类型：
--
作者：
Liu, Xueying;Liu, Zhe;Wang, Peng
DNA单指结合蛋白（DNA binding with One Finger，Dof）是一类植物特异性转录因子，具有高度保守的Dof结构域，包括C2-C2型锌指基序。在这项研究中，我们确定了45 PbDofs梨（Pyrusbretschneideri）。系统发育分析将PbDofs分为8个亚科。通过MEME分析PbDof蛋白的保守基序。D-1亚家族中的PbDofs与拟南芥中的CDFs同源。在这项研究中，我们发现PbDof9.2受生物钟和光周期的调节。PbDof9.2-GFP蛋白定位于细胞核。在拟南芥中过量表达PbDof9.2导致开花时间延迟。PbDof9.2抑制开花时间调节因子FT，并通过促进PbTFL 1a和PbTFL 1b启动子的活性来抑制开花时间。这些结果表明Dof转录因子在植物发育过程中具有保守的功能。
DNA binding with One Finger (Dof) proteins are plant-specific transcription factors with highly conserved Dof domain, including C2-C2 type zinc finger motifs. In this study, we identified 45 PbDofs in pear (Pyrusbretschneideri). PbDofs were classified into eight subfamilies by phylogenetic analysis. Conserved motifs of PbDof proteins were analyzed by MEME. PbDofs in subfamily D-1 werehomologous to CDFs in Arabidopsis. In this study, we showed that PbDof9.2 was regulated by both the circadian clock and photoperiod. PbDof9.2-GFP proteinwas localized in the nucleus. Overexpression of PbDof9.2 in Arabidopsis caused delayed flowering time. PbDof9.2 suppressed the flowering time regulator FT and could repress flowering time by promoting activity of PbTFL1a and PbTFL1b promoter. These results suggest that Doftranscription factors have conserved functions in plant development .