Molecular cloning and characterization of CAPER, a novel coactivator of activating protein-1 and estrogen receptors
Molecular cloning and characterization of CAPER, a novel coactivator of activating protein-1 and estrogen receptors
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DOI：
10.1074/jbc.m110417200
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发表时间：
2002-01-11
影响因子：
4.8
通讯作者：
Lee, JW
中科院分区：
文献类型：
--
作者：
Jung, DJ;Na, SY;Lee, JW
转录共激活子可以连接转录因子和基础转录装置的组件和/或重塑染色质结构。我们基于与最近描述的通用辅活化子激活信号共整合因子-2(ASC-2)的相互作用，分离出一种新的核蛋白。在我们测试的许多转录因子中，该蛋白(作为激活蛋白-1(BAR-1上的(AP)和雌激素受体((ER)上的BAR)的共同激活剂)选择性地结合了AP-1组分c-jun以及ERα和ERβ的雌二醇结合配体结合域。有趣的是，CAPER显示出一种神秘的自主反式激活功能，只有在雌激素结合的内质网存在时才被激活。在共转染中，CAPER通过ERpha、ERbeta和AP-1刺激反式激活。因此，CAPER可能代表了一种更具选择性的转录辅助激活分子，它与一般的辅助激活因子ASC-2一起在体内对AP-1和ER的功能起着关键作用。
Transcriptional coactivators either bridge transcription factors and the components of the basal transcription apparatus and/or remodel the chromatin structures. We isolated a novel nuclear protein based on its interaction with the recently described general coactivator activating signal cointegrator-2 (ASC-2). This protein CAPER (for coactivator of activating protein-1 ((AP) over bar -1) and estrogen receptors ((ER) over bars)) selectively bound, among the many transcription factors we tested, the AP-1 component c-Jun and the estradiol-bound ligand binding domains of ERalpha and ERbeta. Interestingly, CAPER exhibited a cryptic autonomous transactivation function that becomes activated only in the presence of estradiol-bound ER. In cotransfections, CAPER stimulated transactivation by ERalpha, ERbeta, and AP-1. Thus, CAPER may represent a more selective transcriptional coactivator molecule that plays a pivotal role for the function of AP-1 and ERs in vivo in conjunction with the general coactivator ASC-2.