SPI-23 of S. Derby: Role in Adherence and Invasion of Porcine Tissues
SPI-23 of S. Derby: Role in Adherence and Invasion of Porcine Tissues
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DOI：
10.1371/journal.pone.0107857
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发表时间：
2014-09-19
期刊：
影响因子：
3.7
通讯作者：
Woodward, Martin J.
中科院分区：
文献类型：
--
作者：
Hayward, Matthew R.;AbuOun, Manal;Woodward, Martin J.
肠道沙门氏菌血清型德比和姆班达卡是从英国不同的牲畜种群中分离出来的。德比沙门氏菌主要从猪和火鸡中分离出来，姆班达卡沙门氏菌主要从牛和鸡中分离出来。每个血清型的两个分离株的基因组序列比对导致在德比沙门氏菌分离株的染色体序列中发现一个新的推测的沙门氏菌致病岛SPI-23。SPI-23全长37kb，包含42个ORF，其中10个为可能的III型效应蛋白。本研究利用猪空肠来源细胞系IPEC-J2和体外培养的猪空肠和结肠器官，分别研究了德比和姆班达卡沙门氏菌的相关性和侵袭率，以及德比沙门氏菌的组织嗜性。我们发现，德比沙门氏菌侵袭并结合到IPEC-J2单层的数量明显多于姆班达卡沙门氏菌，并且德比沙门氏菌优先附着在猪空肠上，而不是结肠外植体。我们还表明，与结肠外植体相比，SPI-23上的九个基因在空肠中上调的程度更大。此外，我们构建了高度上调的PilV样基因PotR的突变体，发现与亲本菌株相比，它产生了过量的表面菌毛，形成了干扰IPEC-J2单层结合和侵袭的强凝集表型。我们认为，Potr可能在组织趋向性中起作用。
Salmonella enterica serovars Derby and Mbandaka are isolated from different groups of livestock species in the UK. S. Derby is predominantly isolated from pigs and turkeys and S. Mbandaka is predominantly isolated from cattle and chickens. Alignment of the genome sequences of two isolates of each serovar led to the discovery of a new putative Salmonella pathogenicity island, SPI-23, in the chromosome sequence of S. Derby isolates. SPI-23 is 37 kb in length and contains 42 ORFs, ten of which are putative type III effector proteins. In this study we use porcine jejunum derived cell line IPEC-J2 and in vitro organ culture of porcine jejunum and colon, to characterise the association and invasion rates of S. Derby and S. Mbandaka, and tissue tropism of S. Derby respectively. We show that S. Derby invades and associates to an IPEC-J2 monolayer in significantly greater numbers than S. Mbandaka, and that S. Derby preferentially attaches to porcine jejunum over colon explants. We also show that nine genes across SPI-23 are up-regulated to a greater degree in the jejunum compared to the colon explants. Furthermore, we constructed a mutant of the highly up-regulated, pilV-like gene, potR, and find that it produces an excess of surface pili compared to the parent strain which form a strong agglutinating phenotype interfering with association and invasion of IPEC-J2 monolayers. We suggest that potR may play a role in tissue tropism.