Rearing of Triatoma infestans Klug (Het., Reduviidae) in the absence of a live host

Rearing of Triatoma infestans Klug (Het., Reduviidae) in the absence of a live host
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在没有活宿主的情况下饲养致病锥蝽 (Triatoma infestans Klug)(Het.,Reduviidae)

DOI:
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发表时间:
1990
期刊:
影响因子:
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通讯作者:
C. Lazzari
C. Lazzari
中科院分区:
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文献类型:
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作者:
J. Núñez;C. Lazzari

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采用一种可同时饲喂大量昆虫的人工喂食器,分析了恰加斯病病媒克鲁格斑蝽(Triatoma infestans Klug)三龄若虫对不同饵料的狼吞虎咽反应。在4°C或-20°C保存时,柠檬酸血比肝化血更容易被接受。在-20°C下冻结血液和随后的溶血并不影响实验前立即解冻的柠檬酸血的摄入量,但实际上在实验前36小时。在人工喂食器中加入老鼠的气味并没有改善臭虫对食物的反应。不含促吞噬剂的盐水溶液被证明会引起狼吞虎咽。
The gorging response of 3rd‐instar nymphs of the vector of Chagas' disease Triatoma infestans Klug to different diets was analysed using an artificial feeder which allows one to feed a great number of bugs simultaneously. Citrated blood was more acceptable than heparinated blood, either stored at 4°C or at –20°C. Freezing of blood at –20°C and their subsequent haemolysis did not affect the intake of citrated blood when it was defrosted immediately before the experiment, but in fact when this occurred 36 hs before. The addition of mice odours to the artificial feeder did not improve the reaction of the bugs to the food it offer. Saline solutions without phagostimulants proved to evoke gorging.