Localized inhibition in the Drosophila mushroom body.
Localized inhibition in the Drosophila mushroom body.
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作者：
Amin H;Apostolopoulou AA;Suárez-Grimalt R;Vrontou E;Lin AC
许多神经元表现出区室化的活动，其中活动不容易在整个细胞中传播，允许输入和输出局部发生。然而，对于更广泛的神经回路，区室化活动的功能含义往往不清楚。我们解决了这个问题，在果蝇蘑菇体，其主要的神经元，凯尼恩细胞，接受反馈抑制的非尖峰interneuron称为前对侧（APL）神经元。我们使用局部刺激和体积钙成像显示APL抑制Kenyon细胞的树突和轴突，APL和APL对Kenyon细胞的抑制作用的活性都是空间定位的（后者稍微少一点），允许APL差异抑制不同的蘑菇体隔室。将这些结果应用于果蝇半脑连接体预测，单个凯尼恩细胞通过APL抑制自己比抑制其他单个凯尼恩细胞更强烈。这些发现揭示了细胞生理学和详细的网络解剖学如何联合收割机影响电路功能。
Many neurons show compartmentalized activity, in which activity does not spread readily across the cell, allowing input and output to occur locally. However, the functional implications of compartmentalized activity for the wider neural circuit are often unclear. We addressed this problem in the Drosophila mushroom body, whose principal neurons, Kenyon cells, receive feedback inhibition from a non-spiking interneuron called the anterior paired lateral (APL) neuron. We used local stimulation and volumetric calcium imaging to show that APL inhibits Kenyon cells’ dendrites and axons, and that both activity in APL and APL’s inhibitory effect on Kenyon cells are spatially localized (the latter somewhat less so), allowing APL to differentially inhibit different mushroom body compartments. Applying these results to the Drosophila hemibrain connectome predicts that individual Kenyon cells inhibit themselves via APL more strongly than they inhibit other individual Kenyon cells. These findings reveal how cellular physiology and detailed network anatomy can combine to influence circuit function.