The ARID-HMG DNA-binding protein AtHMGB15 is required for pollen tube growth in Arabidopsis thaliana.
The ARID-HMG DNA-binding protein AtHMGB15 is required for pollen tube growth in Arabidopsis thaliana.
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DOI：
10.1111/tpj.12582
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发表时间：
2014-09
期刊：
影响因子：
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通讯作者：
Chuan Xia;Yu-Jiao Wang;Yan Liang;Q. Niu;Xiao-Yun Tan;Liang-Cui Chu;Li-Qun Chen;Xueqin Zhang;D. Ye
中科院分区：
文献类型：
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作者：
Chuan Xia;Yu-Jiao Wang;Yan Liang;Q. Niu;Xiao-Yun Tan;Liang-Cui Chu;Li-Qun Chen;Xueqin Zhang;D. Ye
在开花植物中，雄配子（精子细胞）在雄配子体（花粉粒）中发育，并通过花粉管传递给雌配子进行双受精。因此，花粉管的生长对于繁殖至关重要。控制花粉管生长的机制仍然知之甚少。在这项研究中，我们证明了ARID-HMG DNA结合蛋白AtHMGB 15在花粉管生长中起着重要作用。该蛋白优先在花粉粒和花粉管中表达，并且定位于三细胞花粉粒和花粉管的营养核中。通过Ds插入敲低AtHMGB 15表达导致花粉管生长迟缓，导致结实率显著降低。athmgb 15 -1突变影响了成熟花粉中1686个基因的表达，包括那些参与细胞壁形成和修饰、细胞信号传导和花粉管生长过程中的细胞运输的基因。此外，观察到AtHMGB 15在体外与DNA结合并与转录因子AGL 66和AGL 104相互作用，这是花粉成熟和花粉管生长所需的。这些结果表明，AtHMGB 15功能在花粉管生长通过基因表达的调节。
In flowering plants, male gametes (sperm cells) develop within male gametophytes (pollen grains) and are delivered to female gametes for double fertilization by pollen tubes. Therefore, pollen tube growth is crucial for reproduction. The mechanisms that control pollen tube growth remain poorly understood. In this study, we demonstrated that the ARID-HMG DNA-binding protein AtHMGB15 plays an important role in pollen tube growth. This protein is preferentially expressed in pollen grains and pollen tubes and is localized in the vegetative nuclei of the tricellular pollen grains and pollen tubes. Knocking down AtHMGB15 expression via a Ds insertion caused retarded pollen tube growth, leading to a significant reduction in the seed set. The athmgb15-1 mutation affected the expression of 1686 genes in mature pollen, including those involved in cell wall formation and modification, cell signaling and cellular transport during pollen tube growth. In addition, it was observed that AtHMGB15 binds to DNA in vitro and interacts with the transcription factors AGL66 and AGL104, which are required for pollen maturation and pollen tube growth. These results suggest that AtHMGB15 functions in pollen tube growth through the regulation of gene expression.