Effects of bathing solution on tensile properties of the cornea
Effects of bathing solution on tensile properties of the cornea
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DOI：
10.1016/j.exer.2013.11.017
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发表时间：
2014-03-01
影响因子：
3.4
通讯作者：
Rahimi, Abdolrasol
中科院分区：
文献类型：
--
作者：
Hatami-Marbini, Hamed;Rahimi, Abdolrasol
角膜是一种透明的组织，其主要功能是保护眼睛的内部内容物和折射入射光。角膜的生物力学性质强烈依赖于基质层（水合生物凝胶）的微观结构和组成。单轴条带测试是表征角膜材料参数的方便且被广泛接受的实验技术。已知该方法中样品的含水量取决于浴槽溶液的渗透压。本研究旨在探讨不同的浴溶液对角膜的单轴拉伸材料性能的影响。牛角膜样品的拉伸行为在六种不同的浴溶液中测量，即高渗溶液（12%NaCl溶液）、普通保存等渗溶液（例如磷酸盐缓冲盐水、眼用平衡盐溶液和0.9%NaCl溶液）、低渗溶液（蒸馏水）和中性溶液（矿物油）。观察到浴槽溶液对角膜样品的拉伸行为具有显著影响。特别地，在引起较少溶胀的浴溶液中测试的试样具有显著更硬的拉伸性质。此外，一个简单的数学模型的基础上Voigt复合材料模型开发来表示所测量的溶液依赖的拉伸性能。目前的研究表明，在单轴拉伸实验中应特别注意角膜厚度（水合作用）。它还提供了重要的数据，角膜的拉伸性能，这些信息可以显着提高角膜生物力学的数值预测的准确性。(C)2014爱思唯尔有限公司版权所有。
The cornea is a transparent tissue with the major functions of protecting the inner contents of the eye and refracting incoming light. The biomechanical properties of the cornea strongly depend on the microstructure and composition of the stromal layer, a hydrated bio-gel. The uniaxial strip testing is a convenient and well-accepted experimental technique for characterizing corneal material parameters. It is known that the water content of specimens in this method depends on the osmolality of the bathing solution. The present study was designed to investigate the effects of different bathing solutions on uniaxial tensile material properties of the cornea. The tensile behavior of bovine corneal samples was measured in six different bathing solutions, i.e. hypertonic solution (12% NaCl solution), common preserving isotonic solutions (e.g. phosphate buffer saline, ophthalmic balanced salt solution, and 0.9% NaCl solution), hypotonic solution (distilled water), and neutral solution (mineral oil). It was observed that the bathing solution had significant influence on the tensile behavior of the corneal samples. In particular, the specimens tested in bathing solutions causing less swelling had significantly stiffer tensile properties. Furthermore, a simple mathematical model based on Voigt composite material model was developed to represent the measured solution-dependent tensile properties. The present study suggests that extra attention should be paid to corneal thickness (hydration) in uniaxial tensile experiments. It also provides important data on tensile properties of the cornea; such information could significantly contribute to improving the accuracy of numerical predictions of corneal biomechanics. (C) 2014 Elsevier Ltd. All rights reserved.