Quantitative Proteomic Analysis of Differential Proteins in the Stroma of Nasopharyngeal Carcinoma and Normal Nasopharyngeal Epithelial Tissue
Quantitative Proteomic Analysis of Differential Proteins in the Stroma of Nasopharyngeal Carcinoma and Normal Nasopharyngeal Epithelial Tissue
复制标题
DOI：
10.1002/jcb.22028
复制
发表时间：
2009-03-01
影响因子：
4
通讯作者：
Chen, Zhu-Chu
中科院分区：
文献类型：
--
作者：
Li, Mei-Xiang;Xiao, Zhi-Qiang;Chen, Zhu-Chu
最近的文献强调了基质细胞及其在癌症发生和进展过程中表达的因子的重要性。为了鉴定参与鼻咽癌（NPC）癌变的基质蛋白，我们使用定量蛋白质组学方法结合激光捕获显微切割（LCM）评估了鼻咽癌和正常鼻咽上皮组织（NNET）基质蛋白表达的差异。进行 LCM 分别纯化来自 NPC 和 NNET 的基质细胞。通过二维差异凝胶电泳（2D-DIGE）结合质谱（MS）分析合并的显微解剖肿瘤和正常基质之间的差异蛋白。鉴定出20个差异蛋白，并通过Western blotting和免疫组化分析进一步证实了两个差异蛋白（L-plastin和S100A9）的表达和定位。我们的研究结果将有助于研究间质在鼻咽癌癌变中的作用，以及发现鼻咽癌细胞与其周围微环境之间的相互作用。 J.细胞。生物化学。 106: 570-579, 2009。(C) 2009 Wiley-Liss, Inc.
The importance of stromal cells and the factors that they expressed during cancer initiation and progression have been highlighted by recent literature. To identify the stromal proteins involved in nasopharyngeal carcinoma (NPC) carcinogenesis, we assessed differences in protein expression of the stroma from NPC and normal nasopharyngeal epithelium tissues (NNET) using a quantitative proteomic approach combined with laser capture microdissection (LCM). LCM was performed to purify stromal cells from the NPC and NNET, respectively. The differential proteins between the pooled microdissected tumor and normal stroma were analyzed by two-dimensional difference gel electrophoresis (2D-DIGE) combined with mass spectrometry (MS). Twenty differential proteins were identified, and the expression and location of two differential proteins (L-plastin and S100A9) were further confirmed by Western blotting and immunohistochemical analysis. Our results will be helpful to study the role of stroma in the NPC carcinogenesis, as well as discover the interaction between NPC cells and their surrounding microenvironment. J. Cell. Biochem. 106: 570-579, 2009. (C) 2009 Wiley-Liss, Inc.