Phyllosphere of staple crops under pig manure fertilization, a reservoir of antibiotic resistance genes.
Phyllosphere of staple crops under pig manure fertilization, a reservoir of antibiotic resistance genes.
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DOI：
10.1016/j.envpol.2019.05.098
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发表时间：
2019-09
影响因子：
8.9
通讯作者：
Shuyidan Zhou;Dong Zhu;M. Giles;Xiao-Ru Yang;T. Daniell;R. Neilson;Yong-guan Zhu
中科院分区：
文献类型：
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作者：
Shuyidan Zhou;Dong Zhu;M. Giles;Xiao-Ru Yang;T. Daniell;R. Neilson;Yong-guan Zhu
在中国，由于越来越多地使用牲畜粪便作为施肥手段，抗生素在农业中的普遍使用被认为是一种潜在的公共卫生风险。这样做可能会增加抗生素抗性基因（ARGs）从动物向土壤和植物的转移。本研究对两种主要作物（水稻和小麦）在不同施肥制度下的ARG富集进行了研究。本研究对种植水稻或小麦的土壤微观群落施用无肥料、矿物肥、清洁（减少抗生素使用）和肮脏（目前使用抗生素）猪粪4种处理，研究施肥对各层球中ARGs丰度的影响。对于水稻和小麦，在两个不同的施肥期后采集样本。从所有水稻和小麦样品中共检测到162个独特的ARGs和5个移动遗传元件（MGEs）。在所有水稻和小麦样品中，与不施肥处理相比，添加干净粪便和脏粪便显著提高了ARG丰度（P <0.001），尽管干净粪便对ARG的富集程度低于脏粪便（P <0.001）。不同作物记录的ARG种类不同，小麦样品的ARG多样性高于水稻样品。这些结果表明，当使用干净和肮脏的猪粪进行施肥时，中国的水稻和小麦等主要作物可能是ARGs的储存库。
In China, the common use of antibiotics in agriculture is recognized as a potential public health risk through the increasing use of livestock derived manure as a means of fertilization. By doing so this may increase the transfer of antibiotic resistance genes (ARGs) from animals, to soils and plants. In this study two staple crops (rice and wheat) were investigated for ARG enrichment under differing fertilization regimes. Here, we applied 4 treatments, no fertilizer, mineral fertilizer, clean (reduced antibiotic practice) and dirty (current antibiotic practice) pig manure, to soil microcosms planted with either rice or wheat, to investigate fertilization effects on the abundance of ARGs in the respective phyllospheres. For both rice and wheat, samples were collected after two separate fertilization periods. In total, 162 unique ARGs and 5 mobile genetic elements (MGEs) were detected from all rice and wheat samples. The addition of both clean and dirty manure, enhanced ARG abundance significantly when compared to no fertilizer treatments (P <0.001), though clean manure enriched ARGs to a lesser extent than dirty manure, in all rice and wheat samples (P <0.001). The classes of ARGs recorded were different between crops, with wheat samples having a higher ARG diversity than rice. These results revealed that staple crops in China such as rice and wheat may be a reservoir for ARGs when clean and dirty pig manure is used for fertilization.