DCK is a promising prognostic biomarker and correlated with immune infiltrates in hepatocellular carcinoma
DCK is a promising prognostic biomarker and correlated with immune infiltrates in hepatocellular carcinoma
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DCK 是一种有前景的预后生物标志物，与肝细胞癌的免疫浸润相关
DOI：
10.1186/s12957-020-01953-1
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发表时间：
2020-07-20
影响因子：
3.2
通讯作者：
Wang, Xiaoying
中科院分区：
文献类型：
--
作者：
Song, Danjun;Wang, Yining;Wang, Xiaoying
研究背景脱氧胞苷激酶（DCK）是核苷生物合成途径中的一种酶，可以影响免疫细胞的发育。然而，肝细胞癌（HCC）中DCK的表达与患者预后和肿瘤浸润免疫细胞（TIIC）之间的关系尚不清楚。方法通过Oncomine和肿瘤免疫估计资源（TIMER）数据库分析DCK在HCC中的表达。通过 Kaplan-Meier 绘图仪研究了 DCK 对临床预后的影响，并在基因表达谱交互分析 (GEPIA) 数据库中进行了验证。通过TIMER数据库分析HCC中DCK表达与TIIC之间的相互关系。另外，通过TIMER和GEPIA数据库计算DCK表达与免疫细胞基因标志物的关系。结果与癌旁正常组织相比，肝癌组织中DCK高表达。此外，DCK 的较高表达与 HCC 患者较差的预后相关，并且与早期和分级患者的生存率降低相关。此外，DCK表达与TIIC呈正相关，TIIC包括CD4+和CD8+T细胞、B细胞、单核细胞、肿瘤相关巨噬细胞(TAM)、M1和M2巨噬细胞、中性粒细胞、自然杀伤细胞和树突状细胞。具体而言，DCK 表达水平与多种免疫基因标记物组显着相关，包括 Tregs 和耗尽的 T 细胞。结论这些研究结果表明，DCK 表达与 HCC 患者的患者预后和肿瘤浸润细胞水平相关。此外，DCK水平的升高与Tregs和耗竭相关抑制性受体的标记基因有关，表明DCK在免疫抑制和免疫逃逸中的潜在作用。这些发现表明DCK可以作为一种潜在的新型预后生物标志物并反映HCC患者的免疫浸润状态。
BackgroundDeoxycytidine kinase (DCK), an enzyme in the nucleoside biosynthetic pathway, can affect the development of immune cells. However, the relationships between the expression ofDCK, patient prognosis, and tumor-infiltrating immune cells (TIICs) in hepatocellular carcinoma (HCC) are still unclear.MethodsThe expression ofDCKin HCC was analyzed through the Oncomine and Tumor Immune Estimation Resource (TIMER) databases. The impact ofDCKon clinical prognosis was investigated via the Kaplan-Meier plotter and verified in the Gene Expression Profiling Interactive Analysis (GEPIA) databases. The interrelationships betweenDCKexpression and TIICs in HCC were analyzed by the TIMER database. Additionally, the relationship betweenDCKexpression and immune cell gene markers was calculated through TIMER and GEPIA databases.ResultsCompared with the adjacent normal tissues, high expression ofDCKwas observed in HCC tissues. Also, the higher expression ofDCKwas correlated to poorer prognosis in HCC patients, and it was associated with decreased survival in those with early stage and grade. Moreover,DCKexpression was positively correlated with TIICs, including CD4+and CD8+T cells, B cells, monocytes, tumor-associated macrophages (TAMs), M1 and M2 macrophages, neutrophils, natural killer cells, and dendritic cells. Specifically,DCKexpression levels were significantly associated with diverse immune gene marker sets, including those of Tregs and exhausted T cells.ConclusionThese findings suggest thatDCKexpression is correlated with patient outcomes and tumor infiltration cell levels in HCC patients. Additionally, the increased level ofDCKwas associated with marker genes of Tregs and exhaustion-related inhibitory receptors, suggesting the potential role ofDCKin immunosuppression and immune escape. These findings suggest thatDCKcan function as a potential novel prognostic biomarker and reflect the immune infiltration status in HCC patients.