Considerations towards a roadmap for collection, handling and storage of blood extracellular vesicles
Considerations towards a roadmap for collection, handling and storage of blood extracellular vesicles
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DOI：
10.1080/20013078.2019.1647027
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发表时间：
2019-12-01
影响因子：
16
通讯作者：
Nieuwland, Rienk
中科院分区：
文献类型：
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作者：
Clayton, Aled;Boilard, Eric;Nieuwland, Rienk
对探索存在于体液中的临床相关信息的兴趣日益增加，并且细胞外囊泡（EV）是体液的内在组分（“液体活检”）。在本报告中，我们将重点关注血液。血液不仅含有EV，还含有细胞和非EV颗粒，包括脂蛋白。由于高浓度的可溶性蛋白质和脂蛋白，血液、血浆和血清具有高粘度和密度，这阻碍了EV的浓缩、分离和检测。由于大多数（如果不是所有）EV研究都是单中心研究，因此其临床相关性仍然有限。因此，迫切需要提高EV研究的标准化和可重复性。作为第一步，国际细胞外囊泡学会于2017年11月在伯明翰（英国）组织了一次生物标志物研讨会，在研讨会期间，成立了几个工作组，专注于特定的体液。本报告是血液EV工作组的第一份成果，基于工作组成员对问卷的答复，以发现路线图的轮廓。从回答中可以清楚地看出，大多数受访者赞成以证据为基础的研究、教育、质量控制程序和物理模型，以提高我们对浓缩、分离和检测方法的理解和比较。由于血液是如此复杂的体液，我们假设调查的结果也可能对探索血液以外的体液有价值。
There is an increasing interest in exploring clinically relevant information that is present in body fluids, and extracellular vesicles (EVs) are intrinsic components of body fluids ("liquid biopsies"). In this report, we will focus on blood. Blood contains not only EVs but also cells, and non-EV particles including lipoproteins. Due to the high concentration of soluble proteins and lipoproteins, blood, plasma and serum have a high viscosity and density, which hampers the concentration, isolation and detection of EVs. Because most if not all studies on EVs are single-centre studies, their clinical relevance remains limited. Therefore, there is an urgent need to improve standardization and reproducibility of EV research. As a first step, the International Society on Extracellular Vesicles organized a biomarker workshop in Birmingham (UK) in November 2017, and during that workshop several working groups were created to focus on a particular body fluid. This report is the first output of the blood EV work group and is based on responses by work group members to a questionnaire in order to discover the contours of a roadmap. From the answers it is clear that most respondents are in favour of evidence-based research, education, quality control procedures, and physical models to improve our understanding and comparison of concentration, isolation and detection methods. Since blood is such a complex body fluid, we assume that the outcome of the survey may also be valuable for exploring body fluids other than blood.