Prevotella intermedia fimbriae mediate hemagglutination.
Prevotella intermedia fimbriae mediate hemagglutination.
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中间普雷沃菌菌毛介导血凝。
DOI：
10.1111/j.1399-302x.1996.tb00335.x
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发表时间：
1996
影响因子：
--
通讯作者：
Clark,WB
中科院分区：
文献类型：
--
作者：
Leung,KP;Fukushima,H;Nesbitt,WE;Clark,WB
我们早期的研究表明，临床菌株ofPrevotella intermedia，分离自人类牙周袋，具有各种类型的伞（表面附属物）的超微结构确定。这些细菌具有凝集选定的哺乳动物红细胞的能力。这些细胞表现出的血凝活性可能归因于这些表面结构。选择具有直径为8 nm的菌毛且易于凝集人、猴、羊、兔和小鼠红细胞的菌株17来确定这些菌毛是否具有血凝活性。机械剪切菌毛，通过硫酸铵沉淀浓缩，溶解于含有0.5%脱氧胆酸盐的10 mM Tris-HCl缓冲液中，并通过在10-50%线性蔗糖梯度中的超离心部分纯化。分离的菌毛密度为1.10-1.15 g/ml，超微结构均匀，具有血凝活性。P.用蛋白酶处理减少中间体全细胞和分离的菌毛，并在80°C下加热处理10 min消除。血凝的最佳pH为7.0。在血凝过程中，中间疟原虫全细胞和分离的菌毛与兔红细胞结合，观察结果如下：（a）与兔红细胞孵育后细菌全细胞和分离的菌毛的血凝活性降低，（b）用于吸收的红细胞的血凝活性增加和（B）用于吸收的红细胞的血凝活性增加和（c）P的存在。免疫电镜显示，吸收后红细胞上有中间全细胞和菌毛。兔多克隆抗菌毛抗体的免疫球蛋白G和鼠单克隆抗菌毛抗体的免疫球蛋白G及其Fab片段均为分离的菌毛介导的血凝的有效抑制剂。免疫胶体金标记表明，该单克隆抗体与P.中间菌毛这些结果共同表明，血凝表现出的P。中间体可能归因于菌毛或菌毛相关成分。
Our earlier studies demonstrated that clinical strains ofPrevotella intermedia, isolated from human periodontal pockets, possess various types of fimbria (surface appendages) as determined ultrastructurally. These bacteria have the ability to agglutinate selected mammalian erythrocytes. Hemagglutinating activity exhibited by these cells may be attributable to these surface structures. Strain 17, which posseses fimbriae of 8 nm in diameter and readily agglutinates human, monkey, sheep, rabbit, and mouse erythrocytes was selected to determine whether these fimbriae possessed the hemagglutinating activity. Fimbriae were mechanically sheared, concentrated by ammonium sulfate precipitation, solubilized in 10 mM Tris‐HCl buffer containing 0.5% deoxycholate and partly purified by ultracentrifugation in a 10–50% linear sucrose gradient. Isolated fimbriae banded at a density of 1.10–1.15 g/ml, appeared fairly uniform ultrastructurally, and possessed hemagglutinating activity. The hemagglutinating activity ofP. intermediawhole cells and isolated fimbriae was reduced by treatment with proteases and eliminated by treatment with heat at 80°C for 10 min. The optimal pH for the hemagglutination was 7.0. In the process of hemagglutination,P. intermediawhole cells and isolated fimbriae bound to rabbit erythrocytes as observed by: (a) decrease in the hemagglutinating activity of bacterial whole cells and isolated fimbriae after incubation with rabbit erythrocytes, (b) increase in hemagglutinating activity of the red cells used for absorption and (c) the presence ofP. intermediawhole cells and fimbriae on red cells after absorption as revealed by immunoelectron microscopy. Both the immune immunoglobulin G of the rabbit polyclonal anti‐fimbriae antibody and the immune immunoglobulin G and its Fab fragments of the murine monoclonal anti‐fimbriae antibody were effective inhibitors of hemagglutination mediated by isolated fimbriae. Immunogold labeling showed that the monoclonal antibody bound specifically toP. intermediafimbriae. These results collectively suggest that the hemagglutination exhibited byP. intermediamay be attributable to the fimbriae or the fimbrial‐associated components.