The inhibition of protein translation mediated by AtGCN1 is essential for cold tolerance in Arabidopsis thaliana.
The inhibition of protein translation mediated by AtGCN1 is essential for cold tolerance in Arabidopsis thaliana.
复制标题
AtGCN1 介导的蛋白质翻译抑制对于拟南芥的耐冷性至关重要。
DOI：
10.1111/pce.12826
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发表时间：
2017-01
期刊：
影响因子：
--
通讯作者：
Zhang H
中科院分区：
文献类型：
--
作者：
Wang L;Li H;Zhao C;Li S;Kong L;Wu W;Kong W;Liu Y;Wei Y;Zhu JK;Zhang H
在酵母中，一般控制不可降压因子1(GCN1)与GCN2的相互作用使GCN2在各种胁迫下磷酸化eIF2α(真核细胞翻译起始因子2的α亚基)。在此，我们克隆了拟南芥中GCN1的同源物AtGCN1。我们发现，在水杨酸(SA)、紫外线(UV)、冷应激和氨基酸缺乏的条件下，AtGCN1与GCN2直接相互作用，对eIF2α的磷酸化是必不可少的。与野生型相比，eif2α磷酸化缺陷的两个突变等位基因atgcn1-1和atgcn1-2对冷胁迫表现出更高的敏感性。核糖体结合的RNA图谱显示，atgcn1-1的mRNA翻译状态高于野生型。我们的结果还表明，低温处理降低了Tor突变体幼苗产生紫色下胚轴的倾向。此外，当植物受到冷胁迫时，TOR的激酶活性被瞬时抑制，这表明TOR的抑制是植物响应冷胁迫的另一条重要途径。综上所述，我们的结果表明，atGCN1介导的eIF2α的磷酸化是拟南芥耐冷性所必需的，这是抑制蛋白质翻译起始所必需的。
In yeast, the interaction of General Control Non-derepressible 1 (GCN1) with GCN2 enables GCN2 to phosphorylate eIF2α (the alpha subunit of eukaryotic translation initiation factor 2) under a variety of stresses. Here, we cloned AtGCN1, an Arabidopsis homologue of GCN1. We show that AtGCN1 directly interacts with GCN2 and is essential for the phosphorylation of eIF2α under salicylic acid (SA), ultraviolet (UV), cold stress and amino acid deprivation conditions. Two mutant alleles, atgcn1-1 and atgcn1-2, which are defective in the phosphorylation of eIF2α, showed increased sensitivity to cold stress, compared with the wild type. Ribosome-bound RNA profiles showed that the translational state of mRNA was higher in atgcn1-1 than in the wild type. Our result also showed that cold treatment reduced the tendency of the tor mutant seedlings to produce purple hypocotyls. In addition, the kinase activity of TOR was transiently inhibited when plants were exposed to cold stress, suggesting that the inhibition of TOR is another pathway important for plants to respond to cold stress. In conclusion, our results indicate that the AtGCN1-mediated phosphorylation of eIF2α, which is required for inhibiting the initiation of protein translation, is essential for cold tolerance in Arabidopsis.
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影响因子：
10.5
作者：
Cherkasova, VA;Hinnebusch, AG
通讯作者：
Hinnebusch, AG
影响因子：
11.4
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Sattlegger, E;Hinnebusch, AG
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Sattlegger, E;Hinnebusch, AG
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64.5
作者：
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9.4
作者：
Duan, Yunfeng;Zhang, Wensheng;Li, Xia
通讯作者：
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