Pyrococcus yayanosii sp nov., an obligate piezophilic hyperthermophilic archaeon isolated from a deep-sea hydrothermal vent
Pyrococcus yayanosii sp nov., an obligate piezophilic hyperthermophilic archaeon isolated from a deep-sea hydrothermal vent
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DOI：
10.1099/ijs.0.024653-0
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发表时间：
2011-12-01
影响因子：
2.8
通讯作者：
Prieur, Daniel
中科院分区：
文献类型：
--
作者：
Birrien, Jean-Louis;Zeng, Xiang;Prieur, Daniel
从位于大西洋中脊4100m深处的热液喷口‘Ashadze’中分离到一株专性嗜压厌氧高温古细菌，命名为CH1(T)，在95℃、42兆帕的静水压力下进行了富集和分离。CH1(T)菌株的细胞为高度活动的不规则球形球菌，直径约1~1.5亩，生长温度80~108℃(最适98℃)，压力20~120兆帕(最适52兆帕)。在60-110℃的大气压下没有观察到生长，在pH 6.0-9.5(最适7.5-8.0)和在2.5-5.5%(w/v)的氯化钠(最适3.5%)中观察到生长。菌株CH1(T)是严格厌氧的，生长在复杂的蛋白质底物上，如酵母膏、蛋白胨和酪蛋白，以及蔗糖、淀粉、甲壳素、丙酮酸、乙酸酯和甘油，没有电子受体。基因组DNA的G+C含量为49.0+/-0.5mol%。16S rRNA基因序列分析表明，菌株CH1(T)属于热球菌属。根据其生理特性和核糖体蛋白之间的相似性水平，CH1(T)菌株代表了一个新物种，命名为雅雅诺热球菌。11月是提议的。模式毒株为CH1(T)(=JCM 16557)。该菌株也可从Souchotheque de Bretagne(LMBE目录册)培养收藏(收藏编号3310)获得。
An obligate piezophilic anaerobic hyperthermophilic archaeon, designated strain CH1(T), was isolated from a hydrothermal vent site named 'Ashadze', which is located on the Mid-Atlantic Ridge at a depth of 4100 m. Enrichment and isolation of the strain were carried out at 95 degrees C under a hydrostatic pressure of 42 MPa. Cells of strain CH1(T) were highly motile irregular cocci with a diameter of similar to 1-1.5 mu m. Growth was recorded at 80-108 degrees C (optimum 98 degrees C) and at pressures of 20-120 MPa (optimum 52 MPa). No growth was observed under atmospheric pressures at 60-110 degrees C. Growth was observed at pH 6.0-9.5 (optimum 7.5-8.0) and in 2.5-5.5 % (w/v) NaCl (optimum 3.5%). Strain CH1(T) was strictly anaerobic and grew on complex proteinaceous substrates, such as yeast extract, Peptone, and casein, as well as on sucrose, starch, chitin, pyruvate, acetate and glycerol without electron acceptors. The G+C content of the genomic DNA was 49.0 +/- 0.5 mol%. Analysis of 16S rRNA gene sequences revealed that strain CH1(T) belongs to the genus Pyrococcus. Based on its physiological properties and similarity levels between ribosomal proteins, strain CH1(T) represents a novel species, for which the name Pyrococcus yayanosii sp. nov. is proposed. The type strain is CH1(T) (=JCM 16557). This strain is also available by request from the Souchotheque de Bretagne (catalogue LMBE) culture collection (collection no. 3310).