The oncolytic herpes simplex virus vector, G47Δ , effectively targets tamoxifen-resistant breast cancer cells
The oncolytic herpes simplex virus vector, G47Δ , effectively targets tamoxifen-resistant breast cancer cells
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溶瘤单纯疱疹病毒载体 G47Delta 可有效靶向对他莫昔芬耐药的乳腺癌细胞。
DOI：
10.3892/or.2015.4539
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发表时间：
2016-03-01
期刊：
影响因子：
4.2
通讯作者：
Liu, Renbin
中科院分区：
文献类型：
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作者：
Fan, Jingjing;Jiang, Hua;Liu, Renbin
本研究的目的是建立他莫昔芬耐药细胞系（MCF-7/TAM-R），并研究G47 Delta对该细胞系的体内外治疗作用。在本研究中，MCF-7/TAM-R单克隆细胞亚系的建立后，MCF-7细胞暴露于他莫昔芬21天。然后，将其与未用他莫昔芬处理的野生型MCF-7亚系（MCF-7 W）进行比较。通过细胞增殖、细胞活力、细胞周期和凋亡分析来检测MCF-7/TAM-R细胞的特性。进行体外和体内毒性研究以研究G47 Delta对MCF-7/TAM-R细胞的治疗作用。与MCF-7 W细胞相比，MCF-7/TAM-R细胞具有更高的增殖能力（P
The aim of the present study was to establish a tamoxifen-resistant cell line (MCF-7/TAM-R) and to investigate the therapeutic effect of G47 Delta on this cell line both in vitro and in vivo. In the present study, the MCF-7/TAM-R monoclonal subline was established after exposing MCF-7 cells to tamoxifen for 21 days. Then, it was compared with a wildtype MCF-7 subline (MCF-7W), which was not treated with tamoxifen. Cell proliferation, viability, cell cycle and apoptosis analyses were carried out to examine the characteristics of the MCF-7/TAM-R cells. Both in vitro and in vivo toxicity studies were conducted to investigate the therapeutic effect of G47 Delta on the MCF-7/TAM-R cells. Compared to the MCF-7W cells, we found that the MCF-7/TAM-R cells exhibited a higher proliferation ability (P