Development of Biologically Active GDC for the Treatment of Cerebral Aneurysms
Development of Biologically Active GDC for the Treatment of Cerebral Aneurysms
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开发用于治疗脑动脉瘤的生物活性 GDC
DOI：
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发表时间：
1999
影响因子：
1.7
通讯作者：
Toshiaki Abe
中科院分区：
文献类型：
--
作者：
Y. Murayama;F. Viñuela;Y. Suzuki;Yasutada Akiba;G. Duckwiler;Gobin Yp;M. Kaibara;K. Kurotobi;M. Iwaki;Toshiaki Abe
体外研究：聚苯乙烯培养皿的表面通过以下方式进行处理：1）未离子注入的胶原蛋白涂层，或2）离子注入的胶原蛋白涂层。用能量为150 keV的Ne ~+离子注入，注入剂量为1 × 10 ~（15）。在培养皿上培养牛内皮细胞，并用胰蛋白酶处理来评价细胞的抗脱离性。实验性动脉瘤研究：GDC用1型胶原、纤连蛋白、玻连蛋白、层粘连蛋白或纤维蛋白原包被。然后对这些蛋白质包被的GDC进行离子注入。在28只猪的双侧颈总动脉显微手术构建了56个实验性动脉瘤。用标准GDC或离子植入蛋白质包被的GDC栓塞动脉瘤。在弹簧圈放置后第14天处死动物。显微镜下观察膀胱口。体外实验表明，离子注入可促进内皮细胞增殖，增强细胞粘附力。在栓塞后14天检查的标本上，在宏观和显微镜下观察到离子植入GDC的动脉瘤颈部有更大的纤维组织覆盖，而只有纤维蛋白样薄层覆盖标准GDC表面。这些体外和体内研究表明，离子注入与GDC的蛋白质包被组合改善细胞粘附/增殖。该技术可改善脑动脉瘤的临床结局。
In Vitro Study: The surface of polystyrene dishes were treated either by: 1) collagen coating without ion implantation, or 2) collagen coaling with ion implantation. Ne+implantation was performed on area 2 with fluences of 1 times 1015at an energy of 150 keV. Bovine endothelial cells were cultured on the dishes and the resistance to detachment of cells was evalated with trypsin treatment. Experimental Aneurysm Study: GDCs were coated with either type 1 collagen, fibronectin, vitronectin, laminin or fibrinogen. Ion implantation was then performed on these protein-coated GDCs. 56 experimental aneurysms were constructed microsurgically in the bilateral common carotid arteries of 28 swine. The aneurysms were embolized with standard GDCs or with ion-implanted protein-coated GDCs. The animals were sacrificed at day 14 after coil placement. The aneurysmal orifice was observed microscopically. In vitro study showed that endothelial cell proliferation and strength of cell attachment were accerelated by ion implantation. On specimens examined 14 days post-embolization, greater fibrous tissue coverage at the neck of the aneurysm was observed macroscopically and microscopically with ion implanted GDCs, whereas only a fibrin-like thin layer covered the standard GDC surfaces. These in vitro and in vivo studied indicate that ion implantation combined with protein coating of GDCs improves cellular adhesion/proliferation. This technology may provide an improvement in clinical outcome of cerebral aneurysms.
影响因子：
4.1
作者：
GUGLIELMI, G;VINUELA, F;SEPETKA, I
通讯作者：
SEPETKA, I
电可脱线圈颅内动脉瘤的血管内闭塞：动脉瘤颈大小与治疗结果的相关性。
DOI：
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发表时间：
1994
期刊：
AJNR. American journal of neuroradiology
影响因子：
--
作者：
FernandezZubillaga,A;Guglielmi,G;Viñuela,F;Duckwiler,GR
通讯作者：
Duckwiler,GR