Cross breeding and hybrid identification of sulphite-tolerant hybrids of saccharomyces uvarum
Cross breeding and hybrid identification of sulphite-tolerant hybrids of saccharomyces uvarum
复制标题
葡萄汁酵母耐亚硫酸盐杂种的杂交育种及杂交鉴定
DOI：
10.21548/38-2-1044
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发表时间：
2017-01-01
影响因子：
1.3
通讯作者：
Zhang, H.Y.
中科院分区：
文献类型：
--
作者：
Liu, X.Z.;Zhang, Z.M.;Zhang, H.Y.
属于酵母属的酵母物种在葡萄酒工业中具有巨大的潜力。然而，大多数S.葡萄囊酵母菌株与其它酵母菌株相比，其耐药性较差。为了得到新的S.从3个不同的敏感S. uvarum菌株杂交组合中筛选出21株耐亚硫酸盐且发酵特性良好的候选菌株。uvarum菌株与一个耐亚硫酸盐的菌株。其中10个杂交种是耐亚硫酸盐的，含有双亲的FZFI基因。简单序列重复序列间（ISSR）分析证实了它们的杂交状态，基于6个引物产生了55条清晰且可重复的条带，其中包括32条多态性条带。两个杂交种的指纹完全相同，说明是同一个克隆体。因此，9个不同的新的抗亚硫酸盐的S。获得悬雍垂。所选择的杂交菌株在30 ℃下在含有2 mM亚硫酸钠的长相思葡萄汁中发酵得非常好，发酵性能略有差异。两个菌株（即C13和C21）的表现与耐亚硫酸盐亲本A9和商业S. C13菌株发酵产丙醇、异丁醇和异戊醇最高。这是利用杂交选育耐亚硫酸盐S.葡萄囊菌株
Yeast species belonging to Saccharomyces have great potential for the wine industry. However, the sulphite tolerance of most S. uvarum strains is quite poor compared with that of the other Saccharomyces strains. In order to get new S. uvarum strains with tolerance to sulphite, and also with good fermentation characteristics, 21 candidates were screened from three different crossing combinations of sensitive S. uvarum strains to one sulphite-tolerant strain. Ten of these hybrids were sulphite tolerant and contained the FZFI gene from both parents. Inter-simple sequence repeat (ISSR) analysis confirmed their hybrid status, based on six primers that produced 55 clear and reproducible bands, including 32 that were polymorphic. Two hybrids had identical fingerprints, indicating that it was the same clone. Thus, nine different novel sulphite-resistant hybrids of S. uvarum were obtained. The selected hybrid strains fermented very well at 30 degrees C in Sauvignon Blanc grape juice containing 2 mM of sodium sulphite, with minor differences in fermentation performance. Two strains (namely C13 and C21) performed very similarly to the sulphite-tolerant parent A9 and a commercial S. cerevisiae strain EC1118, and the production of fermentation aromas, namely propanol, isobutanol and isoamyl alcohol by C13 was found to be the highest. This is the first report of using hybridisation to breed the sulphite-tolerant S. uvarum strains.