Improving carotenoids production in yeast via adaptive laboratory evolution
Improving carotenoids production in yeast via adaptive laboratory evolution
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DOI：
10.1016/j.ymben.2013.11.002
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发表时间：
2014-01-01
影响因子：
8.4
通讯作者：
Kao, Katy C.
中科院分区：
文献类型：
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作者：
Reyes, Luis H.;Gomez, Jose M.;Kao, Katy C.
适应性实验室进化是工业相关表型菌株工程的重要工具。传统上，自适应实验室进化是为了提高工业菌株在不同操作条件下的稳健性；然而，由于生长和生存之间需要耦合，应用于增加次生代谢物产量的应用通常会由于所产生化合物的代谢负担或毒性而导致产量下降。在本研究中，通过利用类胡萝卜素的抗氧化特性，成功地应用自适应实验室进化来提高酿酒酵母工程菌株的胡萝卜素产量。使用周期性过氧化氢电击方案的短期进化实验导致类胡萝卜素产量增加3倍(从6毫克/克干细胞重量增加到高达18毫克/克干细胞重量)。随后的转录组分析被用来阐明类胡萝卜素产量增加的分子机制。在所分析的类胡萝卜素高产菌中，与脂肪生物合成和甲羟戊酸生物合成途径相关的基因普遍上调。(C)2013国际新陈代谢工程学会。爱思唯尔公司出版，版权所有。
Adaptive laboratory evolution is an important tool for the engineering of strains for industrially relevant phenotypes. Traditionally, adaptive laboratory evolution has been implemented to improve robustness of industrial strains under diverse operational conditions; however due to the required coupling between growth and survival, its application for increased production of secondary metabolites generally results in decreased production due to the metabolic burden imposed by, or toxicity of, the produced compound.In this study, adaptive laboratory evolution was successfully applied to improve carotenoicls production in an engineered Saccharornyces cerevisine producer strain by exploiting the antioxidant properties of carotenoids. Short-term evolution experiment using periodic hydrogen peroxide shocking schemes resulted in a 3-fold increase in carotenoids production (from 6 mg/g dry cell weight to up to 18 mg/g dry cell weight). Subsequent transcriptome analysis was used to elucidate the molecular mechanisms for increased carotenoids production. Upregulation of genes related with lipid biosynthesis and mevalonate biosynthesis pathways were commonly observed in the carotenoids hyper-producers analyzed. (C) 2013 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.