Micro and macroarchitectural changes at the tibia after botulinum toxin injection in the growing rat
Micro and macroarchitectural changes at the tibia after botulinum toxin injection in the growing rat
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DOI：
10.1016/j.bone.2011.12.023
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发表时间：
2012-04-01
期刊：
影响因子：
4.1
通讯作者：
Chappard, D.
中科院分区：
文献类型：
--
作者：
Bouvard, B.;Mabilleau, G.;Chappard, D.
本研究的目的是分析生长大鼠废用模型中的骨微结构和胫骨宏观结构。 8周龄哥本哈根大鼠右后肢股四头肌肌内注射1.5单位BTX。在左后肢注射盐水作为对照。第 1 天处死 5 只大鼠，代表基线组 (D1)，第 14 天 (D14) 处死 5 只大鼠，第 21 天 (D21) 处死 5 只，第 28 天 (D28) 处死 5 只，第 35 天 (35) 周六处死 5 只。对于每组，使用显微断层扫描测量肌肉表面、骨微结构和宏观结构参数（包括胫骨的长度、宽度和曲率）。第二天就出现瘫痪。在研究期间，左后肢的肌肉表面积、皮质厚度、横截面总面积和长度增长显着增加。在右后肢，肌肉表面积、骨小梁体积和皮质厚度在第 14 天就下降，并伴有皮质孔隙率增加。长度增长与左侧没有差异；横截面总面积没有增加，并且骨干横截面获得更圆的形状。在时间研究期间，左右后肢之间的曲率没有改变。在这种生长动物单侧肌肉麻痹的小鼠模型中，我们发现肌肉快速损失导致宽度增长减少；然而，长度和曲率的增长没有改变。 (C) 2012 Elsevier Inc. 保留所有权利。
The aim of this study was to analyze bone microarchitecture and macroarchitecture of tibia in a disuse model in growing rats. Eight-weeks-old Copenhagen rats were injected intramuscularly with 1.5 units BTX in the quadriceps muscle of the right hind limb. Saline injection was done at the left hind limb to serve as control. Five rats were killed at day 1 and represented the baseline group (D1), 5 rats were killed at day 14 (D14), 5 at day 21 (D21), 5 at day 28 (D28) and Sat day 35 (35). For each group, muscle surface, parameters of bone microarchitecture and macroarchitecture (including length, width and curvature of the tibia) were measured using microtomography. Paralysis occurred as soon as day 2. At the left hind limb, muscle surface area, cortical thickness, cross sectional total area and growth in length significantly increased during the time study. At the right hind limb, muscle surface area, bone trabecular volume, and cortical thickness decreased as soon as day 14 associated with an increased cortical porosity. Growth in length did not differ from left side; cross sectional total area did not increase and the diaphyseal cross section acquired a more rounded shape. There was no modification of the curvature between right and left hind limbs during the time study. In this murine model of unilateral muscle paralysis in growing animals, we showed a rapid muscle loss leading to a decreased growth in width; however growth in length and curvature were unaltered. (C) 2012 Elsevier Inc. All rights reserved.