Production and properties of a laccase isozyme (FvLcc3) from the edible mushroom Flammulina velutipes, which is undetectable under the culture condition for fruiting body formation
Production and properties of a laccase isozyme (FvLcc3) from the edible mushroom Flammulina velutipes, which is undetectable under the culture condition for fruiting body formation
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食用菌金针菇漆酶同工酶（FvLcc3）的制备及性质，在子实体形成培养条件下检测不到该酶
DOI：
10.1093/bbb/zbac027
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Akira Watanabe
中科院分区：
文献类型：
--
作者：
Aylin Cesur;Yuya Nada;Yasuhiko Asada;Akira Watanabe
食用菌金针菇的胞外漆酶同工酶(FvLcc3)在子实体形成的培养条件下检测不到。对FvLcc3进行了纯化，确定FvLcc3为约53 kDa的单体蛋白。FvLCC3对2，2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic酸的催化效率最高(kcat/kM)，其次是2，6-二甲氧基苯酚和愈创木酚，不氧化3，4-二羟基-L苯丙氨酸和l-酪氨酸。
Extracellular laccase isozyme (FvLcc3) from the edible mushroomFlammulina velutipeswas found to be undetectable under the culture condition for fruiting body formation. FvLcc3 was purified and determined to be an approximately 53-kDa monomeric protein. FvLcc3 showed the highest catalytic efficiency (kcat/Km) toward 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) followed by 2,6-dimethoxyphenol and guaiacol and did not oxidize 3,4-dihydroxy-l-phenylalanine andl-tyrosine.