Rat lymphoid cell lines with human T cell leukemia virus production. I. Biological and serological characterization.
Rat lymphoid cell lines with human T cell leukemia virus production. I. Biological and serological characterization.
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大鼠淋巴样细胞系伴有人T细胞白血病病毒的产生。 I.生物学和血清学特征。
DOI：
10.1084/jem.159.4.1105
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发表时间：
1984-04-01
影响因子：
15.3
通讯作者：
Yoshiki, T
中科院分区：
文献类型：
--
作者：
Tateno, M;Kondo, N;Itoh, T;Chubachi, T;Togashi, T;Yoshiki, T
在5-溴-2 '-脱氧尿苷（BrdUrd）存在下，将雌性Wistar-King-Aptekman大鼠的脾细胞、淋巴结细胞和胸腺细胞与短期培养的雄性成人T细胞白血病（ATL）细胞共培养，建立了大鼠淋巴细胞系TARS- 1、TARL-2和TART-1。三个细胞系的细胞遗传学分析显示，雌性大鼠核型为42条染色体。TARS-1和TART-1的表面表型是大鼠T细胞的表型。TARL-2仅对大鼠Ia和白细胞共同抗原呈阳性。细胞系连续产生C型逆转录病毒，人T细胞白血病病毒（HTLV）和表达ATL相关抗原。TARS-1和TART-1可移植到新生的同基因大鼠和裸鼠中，而TARL-2不能。这些结果有力地表明，HTLV不仅永生化，而且在体外转化大鼠T细胞。用TARS-1或TARL-2免疫的成年大鼠产生HTLV特异性抗体。与大鼠血清反应的抗原的生物化学分析显示，它们是两种HTLV特异性多肽，p24和p28。
Cocultivation of spleen cells, lymph node cells, and thymocytes of female Wistar-King-Aptekman rats with short-term cultured male adult T cell leukemia (ATL) cells in the presence of 5-bromo-2'-deoxyuridine (BrdUrd) resulted in the establishment of rat lymphoid cell lines, TARS- 1, TARL-2, and TART-1. Cytogenetic analysis of the three cell lines showed a female rat karyotype with 42 chromosomes. The surface phenotypes of TARS-1 and TART-1 were those of rat T cells. TARL-2 was only positive for rat Ia and leukocyte common antigens. The cell lines continuously produced a type C retrovirus, human T cell leukemia virus (HTLV) and expressed ATL-associated antigens. TARS-1 and TART-1, but not TARL-2 were transplantable into newborn syngeneic rats and nude mice. These results strongly indicate that HTLV not only immortalizes, but also transforms rat T cells in vitro. Adult rats immunized with either TARS-1 or TARL-2 produced antibodies specific for HTLV. The biochemical analysis of the antigens that reacted with rat sera revealed that they are the two HTLV-specific polypeptides, p24 and p28.