TECHNIQUE FOR ENUMERATION OF HETEROTROPHIC AND PHOTOTROPIC NANOPLANKTON, USING EPIFLUORESCENCE MICROSCOPY, AND COMPARISON WITH OTHER PROCEDURES
TECHNIQUE FOR ENUMERATION OF HETEROTROPHIC AND PHOTOTROPIC NANOPLANKTON, USING EPIFLUORESCENCE MICROSCOPY, AND COMPARISON WITH OTHER PROCEDURES
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DOI：
10.1128/aem.46.2.491-498.1983
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发表时间：
1983-01-01
影响因子：
4.4
通讯作者：
CARON, DA
中科院分区：
文献类型：
--
作者：
CARON, DA
描述了一种新方法，其使用荧光素报春花素和落射荧光显微术来计数异养和向光性微型浮游生物（2-20 μ m）。向光微生物与异养生物的区别在于叶绿素a的红色自发荧光。使用单独的过滤器组，其允许可视化的报春花素染色的纳米浮游生物，而不掩蔽叶绿素a荧光，从而允许容易识别的光养细胞。与现有的epifluorescence计数异养和光养nanoplankton技术的比较表明，樱草素提供了更准确的计数这些人口比异硫氰酸荧光素或proflavine技术。准确度与吖啶橙子技术相当，但该方法仅需1个滤膜制备液即可用于光养和异养种群的计数。
A new method is described that uses the fluorochrome primulin and epifluorescence microscopy for the enumeration of heterotrophic and phototropic nanoplankton (2-20 .mu.m). Phototropic microorganisms are distinguished from heterotrophs by the red autofluorescence of chlorophyll a. Separate filter sets are used which allow visualization of the primulin-stained nanoplankton without masking chlorophyll a fluorescence, thus allowing easy recognition of phototrophic cells. Comparison with existing epifluorescence techniques for counting heterotrophic and phototrophic nanoplankton shows that primulin provides more accurate counts of these populations than the fluorescein isothiocyanate or proflavine techniques. Accuracy is comparable to that with the acridine orange technique, but this method requires only 1 filter preparation for the enumeration of both phototrophic and heterotrophic populations.