Total extract and standardized fraction from the stem bark of Butea monosperma have osteoprotective action: evidence for the nonestrogenic osteogenic effect of the standardized fraction
Total extract and standardized fraction from the stem bark of Butea monosperma have osteoprotective action: evidence for the nonestrogenic osteogenic effect of the standardized fraction
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DOI：
10.1097/gme.0b013e3181d0f7f0
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发表时间：
2010-05-01
影响因子：
2.7
通讯作者：
Singh, Divya
中科院分区：
文献类型：
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作者：
Pandey, Rashmi;Gautam, Abnish K.;Singh, Divya
目的：本研究的目的是确定从Butea monosperma中提取的Butea总提取物（BTE）及其丙酮可溶性部分（ASF）对卵巢切除（OVX）大鼠（绝经后骨质流失模型）的骨骼作用。BTE（1.0 g kg（-1）d（-1））和ASF（100 mg kg（-1）d（-1））经口给予成年OVX大鼠12周。假手术组和卵巢切除+溶媒组作为对照。研究了骨密度、类骨质形成（矿物质沉积率和骨形成率）、骨微结构和骨转换/吸收标志物。采用高效液相色谱法分析了给予BTE和ASF的大鼠中的植物雌激素。采用单因素方差分析来检验效果的显著性。结果：与卵巢切除术+溶剂组相比，接受BTE或ASF治疗的OVx大鼠的骨小梁骨密度增加，骨小梁微结构改善。ASF治疗在维持小梁微结构方面比BTE治疗更有效。BTE或ASF处理的OVx大鼠的血清骨钙素和尿1型胶原水平显著低于卵巢切除+载体组。与卵巢切除+赋形剂相比，ASF治疗导致矿物质沉积率和骨形成率增加，而BTE没有这种作用。在促子宫试验中，BTE在成年OVX大鼠中具有轻度雌激素样作用。在未成年大鼠中，BTE表现出雌激素样作用和抗雌激素样作用。ASF无子宫雌激素样作用，也无抗雌激素样作用。植物雌激素的分析显示显着富集cladrin，异芒柄花素，和medicarpin在ASF超过BTE.Conclusions：来自B monosperma，ASF在10倍低于BTE的剂量是有效的，在防止卵巢引起的骨丢失和刺激新骨形成。
Objective: The aim of this study was to determine the skeletal effects of Butea total extract (BTE) and its acetone soluble fraction (ASF) from Butea monosperma, which is rich in methoxyisoflavones, in ovariectomized (OVx) rats, a model for postmenopausal bone loss.Methods: BTE (1.0 g kg(-1) d(-1)) and ASF (100 mg kg(-1) d(-1)) were given orally for 12 weeks to adult OVx rats. The sham-operated and ovariectomy + vehicle groups served as controls. Bone mineral density, osteoid formation (mineral apposition rate and bone formation rate), bone microarchitecture, and bone turnover/resorption markers were studied. Phytoestrogens in rats given BTE and ASF were analyzed by high-performance liquid chromatography. One-way analysis of variance was used to test significance of effects.Results: OVx rats treated with either BTE or ASF exhibited increased bone mineral density in trabecular bones and improved trabecular microarchitecture compared with the ovariectomy + vehicle group. ASF treatment was more efficient than BTE treatment in maintaining trabecular microarchitecture. Serum osteocalcin and urinary type 1 collagen levels in OVx rats treated with either BTE or ASF were significantly lower than those of the ovariectomy + vehicle group. ASF treatment led to increased mineral apposition rate and bone formation rate compared with ovariectomy + vehicle, whereas BTE had no such effect. In the uterotropic assay, BTE was mildly estrogenic in adult OVx rats. In immature rats, BTE exhibited both estrogenicity and antiestrogenicity. ASF had neither uterine estrogenicity nor antiestrogenicity. Analysis of phytoestrogens revealed significant enrichment of cladrin, isoformononetin, and medicarpin in ASF over BTE.Conclusions: Derived from B monosperma, ASF at a 10-fold lower dose than that of BTE was effective in preventing OVx-induced bone loss and stimulated new-bone formation.